Syngap1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:334714 Yang R, et al., Upregulation of SYNGAP1 expression in mice and human neurons by redirecting alternative splicing. Neuron. 2023 Mar 3;
Assay type:	RT-PCR
MGI Accession ID:	MGI:7564425
Gene symbol:	Syngap1
Gene name:	synaptic Ras GTPase activating protein 1 homolog (rat)

Probe:

CH743-CH744

Results

Image: S4B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	P1	TS27: neocortex	Present (b)	Present	Not Specified	C57BL/6		Not Specified
+/+	P1	TS27: neocortex	Present (d)	Present	Not Specified	C57BL/6		Not Specified
+/+	P1	TS27: neocortex	Present (e)	Present	Not Specified	C57BL/6		Not Specified
S/S	P1	TS27: neocortex	Weak (f)	Present	Not Specified	C57BL/6-Syngap1^em2Xiaoz	Syngap1^em2Xiaoz/Syngap1^em2Xiaoz	Not Specified
S/N	P1	TS27: neocortex	Weak (g)	Present	Not Specified	C57BL/6	Syngap1^em1Xiaoz/Syngap1^em2Xiaoz	Not Specified
S/+	P1	TS27: neocortex	Present (h)	Present	Not Specified	C57BL/6-Syngap1^em2Xiaoz	Syngap1^em2Xiaoz/Syngap1⁺	Not Specified
N/+	P1	TS27: neocortex	Weak (i)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1⁺	Not Specified
S/+	P1	TS27: neocortex	Weak (j)	Present	Not Specified	C57BL/6-Syngap1^em2Xiaoz	Syngap1^em2Xiaoz/Syngap1⁺	Not Specified
S/S	P1	TS27: neocortex	Weak (i)	Present	Not Specified	C57BL/6-Syngap1^em2Xiaoz	Syngap1^em2Xiaoz/Syngap1^em2Xiaoz	Not Specified
N/+	P1	TS27: neocortex	Weak (f)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1⁺	Not Specified

Notes:
(a) Larger band, alternative 3' splice site (A3SS)
(b) PSI (percent splice insertion) = 24% A3SS/(total transcripts)
(c) Smaller band
(d) PSI = 25% A3SS/(total transcripts)
(e) PSI = 26% A3SS/(total transcripts)
(f) PSI = 9% A3SS/(total transcripts)
(g) PSI = 7% A3SS/(total transcripts)
(h) PSI = 20% A3SS/(total transcripts)
(i) PSI = 14% A3SS/(total transcripts)
(j) PSI = 16% A3SS/(total transcripts)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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