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Gene Expression Data
Assay Details
Assay
Reference: J:233705 Zhao H, et al., Deletions of Retinoblastoma 1 (Rb1) and Its Repressing Target S Phase Kinase-associated protein 2 (Skp2) Are Synthetic Lethal in Mouse Embryogenesis. J Biol Chem. 2016 May 6;291(19):10201-9
Assay type: Western blot
MGI Accession ID: MGI:7562577
Gene symbol: Cdkn1b
Gene name: cyclin dependent kinase inhibitor 1B
Results
Image: 6E p27 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 26 kDa Genetic BackgroundMutant Allele(s)Sex Note
Rb1lox/lox E13.5 E13.5 TS21: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3TyjNot Specified
Nes-Cre;Rb1lox/lox E13.5 E13.5 TS21: brain Present (a) involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox;Skp2-/- E13.5 E13.5 TS21: brain Strong (a) involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1KinNot Specified
Nes-Cre;Rb1lox/lox;Skp2-/- E13.5 E13.5 TS21: brain Strong (b) involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1Kin, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox E15.5 E15.5 TS23: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3TyjNot Specified
Nes-Cre;Rb1lox/lox E15.5 E15.5 TS23: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox;Skp2-/- E15.5 E15.5 TS23: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1KinNot Specified
Nes-Cre;Rb1lox/lox;Skp2-/- E15.5 E15.5 TS23: brain Present (c) involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1Kin, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox E17.5 E17.5 TS25: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3TyjNot Specified
Nes-Cre;Rb1lox/lox E17.5 E17.5 TS25: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox;Skp2-/- E17.5 E17.5 TS25: brain Present involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1KinNot Specified
Nes-Cre;Rb1lox/lox;Skp2-/- E17.5 E17.5 TS25: brain Present (d) involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1Kin, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Notes:
(a) Increased expression compared with Rb1<lox/lox>.
(b) Skp2-/- did not correct the increased expression but instead increased the expression further.
(c) Slightly increased expression compared with Rb1<lox/lox>.
(d) Similar as in the controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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