Casp3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:233705 Zhao H, et al., Deletions of Retinoblastoma 1 (Rb1) and Its Repressing Target S Phase Kinase-associated protein 2 (Skp2) Are Synthetic Lethal in Mouse Embryogenesis. J Biol Chem. 2016 May 6;291(19):10201-9
Assay type:	Western blot
MGI Accession ID:	MGI:7562554
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved caspase-3 (Asp175) (5A1E; #9664)
Assay notes:	The antibody used recognizes the activated form of the protein.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	17 kDa (a)	Genetic Background	Mutant Allele(s)	Sex	Note
Rb1^lox/lox E13.5	E13.5	TS21: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj	Not Specified
Nes-Cre;Rb1^lox/lox E13.5	E13.5	TS21: brain	Weak (c)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Rb1^lox/lox;Skp2-/- E13.5	E13.5	TS21: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin	Not Specified
Nes-Cre;Rb1^lox/lox;Skp2-/- E13.5	E13.5	TS21: brain	Strong (d)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Rb1^lox/lox E15.5	E15.5	TS23: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj	Not Specified
Nes-Cre;Rb1^lox/lox E15.5	E15.5	TS23: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Rb1^lox/lox;Skp2-/- E15.5	E15.5	TS23: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin	Not Specified
Nes-Cre;Rb1^lox/lox;Skp2-/- E15.5	E15.5	TS23: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Rb1^lox/lox E17.5	E17.5	TS25: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj	Not Specified
Nes-Cre;Rb1^lox/lox E17.5	E17.5	TS25: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Rb1^lox/lox;Skp2-/- E17.5	E17.5	TS25: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin	Not Specified
Nes-Cre;Rb1^lox/lox;Skp2-/- E17.5	E17.5	TS25: brain	Ambiguous (b)	involves: 129 * C57BL/6J * FVB	Rb1^tm3Tyj/Rb1^tm3Tyj, Skp2^tm1Kin/Skp2^tm1Kin, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.

Notes:
(a) activated form
(b) Trace or absent expression.
(c) Increased expression compared with the Rb1<lox/lox> control.
(d) Skp2-/- did not correct the increased expression but instead increased the expression further.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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