Antibody:	Anti-Sox-2 (E-4): sc-365823
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	1A WT Sox2/Pax6 , 1A WT Sox2	Expression was detected in the apical radial glial progenitor cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	1A DKO Sox2 , 1A DKO Sox2/Pax6	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	1A WT Sox2/Tbr2	Expression was detected in the apical radial glial progenitor cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	1A DKO Sox2/Tbr2	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	EV1A Anti-Sox2	Expression was detected in proliferating ventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A WT Sox2/Pax6 , EV2A WT Sox2	Expression was detected in the apical radial glial progenitor cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A Alk-/- Sox2/Pax6 , EV2A Alk-/- Sox2	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A Ltk-/- Sox2/Pax6 , EV2A Ltk-/- Sox2	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A WT Sox2/Tbr2	Expression was detected in the apical radial glial progenitor cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A Alk-/- Sox2/Tbr2	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	forebrain radial glial cell	Present	Regionally restricted	EV2A Ltk-/- Sox2/Tbr2	Expression was detected in the apical radial glial progenitor cells. A marked reduction in the Sox2-positive multipotent progenitors was observed.