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Gene Expression Data
Assay Details
Assay
Reference: J:238708 Gonen N, et al., Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. PLoS Genet. 2017 Jan;13(1):e1006520
Assay type: RT-PCR
MGI Accession ID: MGI:7537342
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Probe: Sox9-pG, Sox9-pH
Probe preparation: labelled with SYBR green
Assay notes: Two primer sets were listed: MGI:3706532 and MGI:3510977. The set used in this assay was not specified. Quantitative RT-PCR. Relative expression was normalized to Tbp. Note: the last 2 columns were light grey (indicating XX), but they were labeled as XY.
Results
Image: S5A Sox9 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
XY WT E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA Not Specified Not Specified
XY TESCO +/- E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA involves: C57BL/6 * C57BL/6J * CBA Sox9em1Rlb/Sox9+Not Specified
XY TESCO -/- E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA involves: C57BL/6 * C57BL/6J * CBA Sox9em1Rlb/Sox9em1RlbNot Specified
XX WT E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA Not Specified Not Specified
XY TESCO +/- E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA involves: C57BL/6 * C57BL/6J * CBA Sox9em1Rlb/Sox9+Not Specified
XY TESCO -/- E12.5 TS20: gonad
TS20: mesonephros 		Present 0.1-0.2 µg; total RNA involves: C57BL/6 * C57BL/6J * CBA Sox9em1Rlb/Sox9em1RlbNot Specified
Notes:
(a) Gonad-mesonephros pairs at 12.5 dpc showed a decrease in Sox9 mRNA levels with XY TESCO+/- and TESCO-/- gonads showing approximately 81% and 73% of wild type levels, respectively.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory