About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type: RT-PCR
MGI Accession ID: MGI:7524636
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Probe: Mm00441712_s1
Probe preparation: labelled with FAM
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 4A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
E11.25 +/+ XY AKR E11.25 TS19: gonad Present Not Specified; total RNA C57BL/6JEi-Chr YAKR/J/EiJ Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.25 Sf1+/- XY AKR E11.25 TS19: gonad Present (a) Not Specified; total RNA B6.Cg-Nr5a1tm1Klp Chr YAKR/J Nr5a1tm1Klp/Nr5a1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.25 Wt1+/- XY AKR E11.25 TS19: gonad Present (b) Not Specified; total RNA B6.Cg-Wt1tm1Jae Chr YAKR/J Wt1tm1Jae/Wt1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.5 +/+ XY AKR E11.5 TS19: gonad Present Not Specified; total RNA C57BL/6JEi-Chr YAKR/J/EiJ Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.5 Sf1+/- XY AKR E11.5 TS19: gonad Present (b) Not Specified; total RNA B6.Cg-Nr5a1tm1Klp Chr YAKR/J Nr5a1tm1Klp/Nr5a1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.5 Wt1+/- XY AKR E11.5 TS19: gonad Present (b) Not Specified; total RNA B6.Cg-Wt1tm1Jae Chr YAKR/J Wt1tm1Jae/Wt1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.75 +/+ XY AKR E11.75 TS20: gonad Present Not Specified; total RNA C57BL/6JEi-Chr YAKR/J/EiJ Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.75 Sf1+/- XY AKR E11.75 TS20: gonad Present (c) Not Specified; total RNA B6.Cg-Nr5a1tm1Klp Chr YAKR/J Nr5a1tm1Klp/Nr5a1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E11.75 Wt1+/- XY AKR E11.75 TS20: gonad Present (c) Not Specified; total RNA B6.Cg-Wt1tm1Jae Chr YAKR/J Wt1tm1Jae/Wt1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E12.0 +/+ XY AKR E12.0 TS20: gonad Present Not Specified; total RNA C57BL/6JEi-Chr YAKR/J/EiJ Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E12.0 Sf1+/- XY AKR E12.0 TS20: gonad Present (a) Not Specified; total RNA B6.Cg-Nr5a1tm1Klp Chr YAKR/J Nr5a1tm1Klp/Nr5a1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
E12.0 Wt1+/- XY AKR E12.0 TS20: gonad Present (a) Not Specified; total RNA B6.Cg-Wt1tm1Jae Chr YAKR/J Wt1tm1Jae/Wt1+Male Gonad/mesonephros complexes were used, but Sry is not expressed in the mesonephros.
Notes:
(a) Not significantly different compared with the wild-type.
(b) Significantly reduced expression compared with the +/+ B6 XY<AKR>.
(c) Significantly reduced expression (less than 50%) compared with the +/+ B6 XY<AKR>.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory