Gene Expression Data
Assay Details
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Assay
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Reference:
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J:183829
Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7524460
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Gene symbol:
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Wnt4
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Gene name:
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wingless-type MMTV integration site family, member 4
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Results
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Specimen
2A +/+ XX:
embryonic day 13.5
(more  )
Specimen
2A +/+ XX:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B +/+ XY AKR:
embryonic day 13.5
(more )
Specimen
2B +/+ XY AKR:
(close )
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Genetic Background:
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B6.Cg-Chr YAKR/J
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: testis |
Present |
Regionally restricted |
2B
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Expressed at the anterior and/or posterior poles in the regions without cords, but not in the central region containing cords.
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Specimen
2C Wt1+/- XX:
embryonic day 13.5; Wt1tm1Jae/Wt1 +
(more )
Specimen
2C Wt1+/- XX:
(close )
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Genetic Background:
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B6.Cg-Wt1tm1Jae
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Mutant Allele(s):
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Wt1tm1Jae/Wt1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mesonephros |
Present |
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2C
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Reduced expression compared with the wild-type.
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| TS21: ovary |
Present |
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2C
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Reduced expression compared with the wild-type.
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Specimen
2D Wt1+/- XY AKR:
embryonic day 13.5; Wt1tm1Jae/Wt1 +
(more )
Note: Gonads differentiated as ovotestes.
Specimen
2D Wt1+/- XY AKR:
(close )
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Genetic Background:
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B6.Cg-Wt1tm1Jae Chr YAKR/J
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Mutant Allele(s):
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Wt1tm1Jae/Wt1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Gonads differentiated as ovotestes.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Regionally restricted |
2D
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Expression was detected at the anterior and/or posterior poles.
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Specimen
not shown Sf1+/- XY AKR:
embryonic day 13.5; Nr5a1tm1Klp/Nr5a1 +
(more )
Note: Gonads differentiated as ovotestes.
Specimen
not shown Sf1+/- XY AKR:
(close )
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Genetic Background:
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B6.Cg-Nr5a1tm1Klp Chr YAKR/J
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Gonads differentiated as ovotestes.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Regionally restricted |
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Expression was detected at the anterior and/or posterior poles.
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Specimen
2E Sf1+/- XX:
embryonic day 13.5; Nr5a1tm1Klp/Nr5a1 +
(more )
Specimen
2E Sf1+/- XX:
(close )
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Genetic Background:
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B6.Cg-Nr5a1tm1Klp
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ovary |
Present |
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2E
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Reduced expression compared with the wild-type.
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| TS21: mesonephros |
Present |
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2E
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Similar as in the wild-type.
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Specimen
2F Sf1+/- XY AKR:
embryonic day 13.5; Nr5a1tm1Klp/Nr5a1 +
(more )
Note: Gonads that developed as ovaries.
Specimen
2F Sf1+/- XY AKR:
(close )
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Genetic Background:
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B6.Cg-Nr5a1tm1Klp Chr YAKR/J
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Gonads that developed as ovaries.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Strong |
Widespread |
2F
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Developed as ovaries, in a pattern similar to XX ovaries.
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Specimen
not shown Wt1+/- XY AKR:
embryonic day 13.5; Wt1tm1Jae/Wt1 +
(more )
Note: Gonads that developed as ovaries.
Specimen
not shown Wt1+/- XY AKR:
(close )
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Genetic Background:
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B6.Cg-Wt1tm1Jae Chr YAKR/J
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Mutant Allele(s):
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Wt1tm1Jae/Wt1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Gonads that developed as ovaries.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Strong |
Widespread |
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Developed as ovaries, in a pattern similar to XX ovaries.
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Analysis Tools