Foxl2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7524449
Gene symbol:	Foxl2
Gene name:	forkhead box L2

Antibody:	Anti-Foxl2 (C terminus)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1A ©

Specimen 1A Wt1+/+ XX: embryonic day 13.5 (more

)
Note: Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: mesenchymal stroma of ovary	granulosa cell	Present		1A

1B ©

Specimen 1B Wt1+/+ XY AKR: embryonic day 13.5 (more

)
Note: Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Level	Pattern	Image	Note
TS21: gonad	Present	Single cells	1B	Expressed in isolated cells within regions containing testicular cords.

1C ©

Specimen 1C Wt1+/- XY AKR: embryonic day 13.5; Wt1^tm1Jae/Wt1⁺ (more

)
Note: Gonads differentiated as ovotestes. Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: gonad	granulosa cell	Present	Regionally restricted	1C	Expressed at the poles.

1D ©

Specimen 1D Sf1+/+ XX: embryonic day 13.5 (more

)
Note: Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: mesenchymal stroma of ovary	granulosa cell	Present		1D

1E ©

Specimen 1E Sf1+/+ XY AKR: embryonic day 13.5 (more

)
Note: Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Level	Pattern	Image	Note
TS21: gonad	Present	Scattered	1E	Expressed in isolated cells within regions containing testicular cords.

1F ©

Specimen 1F Sf1+/- XY AKR: embryonic day 13.5; Nr5a1^tm1Klp/Nr5a1⁺ (more

)
Note: Gonads differentiated as ovaries. Double labeled: green - Foxl2; red - Amh (assay MGI:7524455).

Structure	Level	Pattern	Image	Note
TS21: gonad	Strong		1F	Developed as ovaries, in a pattern similar to XX ovaries.

S2A left ©

Specimen S2A left: embryonic day 13.5; X/Sry^AKR/J Sry^RIII (more

)
Note: Triple labeled: green - Foxl2; red - Amh (assay MGI:7524455); blue - Pecam1 (assay MGI:7525435).

Structure	Level	Pattern	Image	Note
TS21: gonad	Present	Regionally restricted	S2A left	Expressed in the poles.

S2A right ©

Specimen S2A right: embryonic day 13.5; X/Sry^AKR/J Sry^RIII (more

)
Note: Triple labeled: green - Foxl2; red - Amh (assay MGI:7524455); blue - Pecam1 (assay MGI:7525435).

Structure	Level	Pattern	Image	Note
TS21: gonad	Present	Single cells	S2A right	Expressed in isolated cells within regions containing testicular cords.

Specimen not shown Wt1+/- XY AKR: embryonic day 13.5; Wt1^tm1Jae/Wt1⁺ (more

)
Note: Gonads differentiated as ovaries.

Structure	Level	Pattern	Image	Note
TS21: gonad	Strong			Developed as ovaries, in a pattern similar to XX ovaries.

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