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Gene Expression Data
Assay Details
Assay
Reference: J:92061 Eng SR, et al., Coordinated regulation of gene expression by Brn3a in developing sensory ganglia. Development. 2004 Aug;131(16):3859-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7521967
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Antibody: anti-Brn3a
Results
Specimen 2D Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Gal (assay MGI:7521968).
Structure Level Pattern Image Note
TS21: trigeminal ganglion Present Widespread 2D Expression co-localized with Gal in majority of trigeminal neurons.
Specimen 2E Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Th (assay MGI:7521970).
Structure Level Pattern Image Note
TS21: trigeminal ganglion Present Widespread 2E Expression in majority of trigeminal neurons, some of which also express Th.
Specimen 4A Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Calb2 (assay MGI:7521972).
Structure Level Pattern Image Note
TS21: hindbrain Present Regionally restricted 4A Expression characterize distinct populations of developing neurons in thalamus, midbrain and hindbrain.
TS21: midbrain Present Regionally restricted 4A Expression characterize distinct populations of developing neurons in thalamus, midbrain and hindbrain.
TS21: thalamus Present Regionally restricted 4A Expression characterize distinct populations of developing neurons in thalamus, midbrain and hindbrain.
Specimen 4B Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Calb2 (assay MGI:7521972).
Structure Level Pattern Image Note
TS21: hindbrain Present Regionally restricted 4B Authors report distinct expression of Pou4f1 and Calb2 - expression in adjacent but non-overlapping cell populations.
TS21: midbrain Present Regionally restricted 4B Authors report distinct expression of Pou4f1 and Calb2 - expression in adjacent but non-overlapping cell populations.
Specimen 4C Brn3a: embryonic day 13.5; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Note: Double labeled: red - Pou4f1; green - Calb2 (assay MGI:7521972).
Structure Level Pattern Image Note
TS21: midbrain Absent 4C
Specimen 4D Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Calb2 (assay MGI:7521972).
Structure Level Pattern Image Note
TS21: retina Present Regionally restricted 4D Expresion overlapping with Calb2. Subset of neurons express both Pou4f1 and Calb2.
Specimen 4F Brn3a: embryonic day 13.5; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Note: Double labeled: red - Pou4f1; green - Calb2 (assay MGI:7521972).
Structure Level Pattern Image Note
TS21: retina Absent 4F
Specimen 4G Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Sst (assay MGI:7521974).
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted 4G Distinct pattern of expression compared to Sst.
Specimen 4H Brn3a: embryonic day 13.5; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Note: Double labeled: red - Pou4f1; green - Sst (assay MGI:7521974).
Structure Level Pattern Image Note
TS21: retina Absent 4H
Specimen 4I Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Sst (assay MGI:7521974).
Structure Level Pattern Image Note
TS21: retina Present Regionally restricted 4I Expression co-localized with Sst in subset of ganglion cells.
Specimen 4K Brn3a: embryonic day 13.5; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Note: Double labeled: red - Pou4f1; green - Sst (assay MGI:7521974).
Structure Level Pattern Image Note
TS21: retina Absent 4K
Specimen 4L/M Brn3a: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Th (assay MGI:7521970).
Structure Level Pattern Image Note
TS21: midbrain tegmentum Present Regionally restricted 4M , 4L In ventral tegmental area and tegmentum, expression in developing neurons distinct from that of Sst.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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