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Gene Expression Data
Assay Details
Assay
Reference: J:275851 Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
Assay type: RNA in situ
MGI Accession ID: MGI:7496098
Gene symbol: Xist
Gene name: inactive X specific transcripts
Probe: allele-specific Xist probe
Probe preparation: Antisense labelled with other - see notes RNA
Visualized with: Fluorescence
Assay notes: M. musculus-specific oligos were labeled with Quasar 570 (red) and M. molossinus-specific oligos labeled with Quasar 670 (white).
Results
Specimen 6A fl/+ female: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Present 6A fl/+ Xp inset , 6A fl/+ Xp , 6A fl/+ Xm , 6A fl/+ Xm inset , 6A fl/+ merge , 6A fl/+ merge inset Expressed from only the paternal-X (white).
Specimen 6A m-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl;Zp3-Cre Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Present 6A m-/- merge , 6A m-/- Xp , 6A3 , 6A2 , 6A1 , 6A m-/- Xm Mosaic distribution of Xist RNA expression and coating: some expressed from and coating of the maternal-X (red), others from and coating of the paternal-X (white), or from both the maternal and paternal X-chromosomes.
Specimen 6-S1 bottom female: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Present 6-S1B lower right inset , 6-S1B lower left inset , 6-S1B lower left , 6-S1B lower right Expressed from only the paternal-X (white).
Specimen 6-S1 top female: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus molossinus-derived dam with a JF1 Mus musculus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Present 6-S1B upper left , 6-S1B upper right inset , 6-S1B upper left inset , 6-S1B upper right Expressed from only the paternal-X (red).
Specimen 6B fl/+ male: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Absent 6B fl/+ Xp inset , 6B fl/+ Xp , 6B fl/+ merge , 6B fl/+ merge inset , 6B fl/+ Xm , 6B fl/+ Xm inset
Specimen 6B m-/- male: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl;Zp3-Cre Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS4: blastocyst Present 6B m-/- Xm , 6B m-/- Xp inset , 6B m-/- merge , 6B m-/- Xp , 6B m-/- merge inset , 6B m-/- Xm inset Ectopic expression from and coating of their sole maternally-inherited X-chromosome in approximately 50% of nuclei.
Specimen 7A fl/+ 8-cell female: embryonic day 2.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 7A upper fl/+ inset , 7A lower fl/+ inset , 7A middle fl/+ , 7A lower fl/+ , 7A upper fl/+ , 7A middle fl/+ inset Expressed from and coated only the paternal X-chromosome (white).
Specimen 7A m-/- 8-cell female: embryonic day 2.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl;Zp3-Cre Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 7A middle m-/- inset , 7A middle m-/- , 7A lower m-/- inset , 7A lower m-/- , 7A upper m-/- , 7A upper m-/- inset A high percentage of nuclei with Xist RNA expression and coating of both X-chromosomes (red and white).
Specimen 7B fl/+ 8-cell male: embryonic day 2.5; Eedtm1c(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 7B upper fl/+ , 7B lower fl/+ , 7B upper fl/+ inset , 7B middle fl/+ , 7B middle fl/+ inset , 7B lower fl/+ inset
Specimen 7B m-/- 8-cell male: embryonic day 2.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: embryo from an Eed fl/fl;Zp3-Cre Mus musculus-derived dam with a JF1 Mus molossinus-derived sire
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 7B upper m-/- inset , 7B upper m-/- , 7B middle m-/- inset , 7B lower m-/- inset , 7B lower m-/- , 7B middle m-/- Almost every nucleus exhibited ectopic Xist expression from and coating of the maternally-inherited X-chromosome (red).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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