Eed^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eed^{tm1d(EUCOMM)Wtsi}
• Name: embryonic ectoderm development; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7398609
• Gene: Eed Location: Chr7:89603862-89630184 bp, - strand Genetic Position: Chr7, 50.42 cM
• Alliance: Eed^{tm1d(EUCOMM)Wtsi} page
• IMPC: Eed gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275851
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_gt1 cassette was inserted at position 89616630 of Chromosome 7 upstream of the exon 7 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the exon 7 at position 89617432. Exon 7 was thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Cre-mediated recombination removed the floxed exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:275851)

Expression

In Mice Carrying this Mutation:

37 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eed Mutation: 117 strains or lines available

References

• Original: J:275851 Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
• All: 1 reference(s)