Gene Expression Data
Assay Details
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Assay
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Reference:
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J:275851
Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7495898
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Gene symbol:
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Xist
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Gene name:
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inactive X specific transcripts
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Probe:
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Xist probe7
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Results
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Specimen
1A female:
embryonic day 3.5; Tg(CAG-EGFP)D4Nagy/0
(more  )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Specimen
1A female:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Tg(CAG-EGFP)D4Nagy/0
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
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Specimen
1A male:
embryonic day 3.5
(more )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Specimen
1A male:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
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Specimen
1B +/-:
embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/Eed +
(more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Specimen
1B +/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eed+
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Age:
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embryonic day 3.0-3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
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Specimen
1B -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.0-3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
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Specimen
3D fl/fl:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3D -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
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Specimen
3-S1D fl/fl male:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3-S1D fl/- or -/- male:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/?
(more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Specimen
3-S1D fl/- or -/- male:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/?
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
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Specimen
4A fl/fl female:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
4A m-/- female:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed +
(more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
Specimen
4A m-/- female:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eed+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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4A middle right
,
4A middle
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A small percentage of nuclei with H3K27me3 enrichment coinciding with the Xist RNA coat, likely due to the expression of zygotic Eed.
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Specimen
4A mz-/- female:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/ Eedtm1d(EUCOMM)Wtsi
(more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Specimen
4A mz-/- female:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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4A lower
,
4A lower right
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But there was no nuclei with H3K27me3 enrichment coinciding with the Xist RNA coat, likely due to no expression of zygotic Eed.
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Specimen
5A m-/- female:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed +
(more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
Specimen
5A m-/- female:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eed+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
|
|
Note:
|
Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
|
Specimen
5A mz-/- female:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/ Eedtm1d(EUCOMM)Wtsi
(more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Specimen
5A mz-/- female:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
|
Specimen
5B mz-/- male:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/ Eedtm1d(EUCOMM)Wtsi
(more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in the female germline.
Specimen
5B mz-/- male:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in the female germline.
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Specimen
5B mz-/- male delayed:
embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/ Eedtm1d(EUCOMM)Wtsi
(more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Specimen
5B mz-/- male delayed:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
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