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Gene Expression Data
Assay Details
Assay
Reference: J:275851 Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
Assay type: RNA in situ
MGI Accession ID: MGI:7495898
Gene symbol: Xist
Gene name: inactive X specific transcripts
Probe: Xist probe7
Probe preparation: Antisense labelled with fluorescein RNA
Results
Specimen 1A female: embryonic day 3.5; Tg(CAG-EGFP)D4Nagy/0 (more )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present 1A lower Xist female , 1A Xist female Coated the paternal-X.
Specimen 1A male: embryonic day 3.5 (more )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1A lower Xist male , 1A Xist male
Specimen 1B +/-: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 1B +/- Xist , 1B +/- lower Xist Coated the paternal-X.
Specimen 1B -/-: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 1B -/- lower Xist , 1B -/- Xist Coated the paternal-X.
Specimen 3D fl/fl: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS4: blastocyst Present 3D fl/fl Xist , 3D right fl/fl Xist
Specimen 3D -/-: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 3D right -/- Xist , 3D -/- Xist Similar as in the control.
Specimen 3-S1D fl/fl male: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS4: blastocyst Absent 3-S1D fl/fl Xist right , 3-S1D fl/fl Xist
Specimen 3-S1D fl/- or -/- male: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Absent 3-S1D fl/- or -/- Xist , 3-S1D fl/- or -/- Xist right
Specimen 4A fl/fl female: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS4: blastocyst Present 4A upper , 4A upper right
Specimen 4A m-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 4A middle right , 4A middle A small percentage of nuclei with H3K27me3 enrichment coinciding with the Xist RNA coat, likely due to the expression of zygotic Eed.
Specimen 4A mz-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 4A lower , 4A lower right But there was no nuclei with H3K27me3 enrichment coinciding with the Xist RNA coat, likely due to no expression of zygotic Eed.
Specimen 5A m-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5A upper left , 5A upper left enlarged Most nuclei displayed a single Xist RNA coat. A subset displayed Xist RNA coating of both X-chromosomes.
Specimen 5A mz-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5A lower left , 5A lower left enlarged Most nuclei displayed a single Xist RNA coat. A subset displayed Xist RNA coating of both X-chromosomes.
Specimen 5B mz-/- male: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in the female germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5B upper left enlarged , 5B upper left A subset of nuclei (few or no nuclei) exhibited ectopic Xist RNA coating of their sole, maternally-inherited X-chromosome.
Specimen 5B mz-/- male delayed: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5B lower left , 5B lower left enlarged A subset of nuclei (in much higher proportions, 20-60%) exhibited ectopic Xist RNA coating of their sole, maternally-inherited X-chromosome.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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