Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		1B
TS20: midbrain roof plate	Present	Regionally restricted	1B	Expressed in the tectum.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		1C	Similar as in the heterozygous.
TS20: midbrain roof plate	Present	Regionally restricted	1C	Expressed in the tectum. Similar as in the heterozygous.

Structure	Level	Pattern	Image	Note
TS22: mammillary area	Present		1E
TS22: hypothalamus	Present	Regionally restricted	1E	Expressed in the ventrolateral region.

Structure	Level	Pattern	Image	Note
TS22: mammillary area	Present		1F
TS22: hypothalamus	Present	Regionally restricted	1F	Expressed in the ventrolateral region.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord lumen	ependymal cell	Present		1H
TS22: spinal cord ventral horn		Present	Regionally restricted	1H	Expressed in a subset of ventral cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord lumen	ependymal cell	Present		1I
TS22: spinal cord ventral horn		Present	Regionally restricted	1I	Expressed in a subset of ventral cells.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Widespread	1J left	Expressed throughout the neuroepithelium.
TS17: brain	Present	Regionally restricted	1J left	Expressed throughout the neuroepithelium, posterior to the future telencephalon.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Widespread	1J right	Expressed throughout the neuroepithelium.
TS17: brain	Present	Regionally restricted	1J right	Expressed throughout the neuroepithelium, posterior to the future telencephalon.

Structure	Level	Pattern	Image	Note
TS26: hypothalamus lateral zone	Present		1K left

Structure	Level	Pattern	Image	Note
TS26: hypothalamus lateral zone	Present		1K right	Smaller expression domain compared with the heterozygous.

Structure	Level	Pattern	Image	Note
TS26: pretectum	Present		1L left

Structure	Level	Pattern	Image	Note
TS26: pretectum	Present		1L right	Smaller expression domain compared with the heterozygous.

Structure	Level	Pattern	Image	Note
TS26: spinal cord ventral horn	Present	Regionally restricted	3A	Expressed in the entire ventromedial spinal cord, and ventrolateral spinal cord at lumbar levels.

Structure	Level	Pattern	Image	Note
TS26: spinal cord lateral wall	Present	Regionally restricted	3B	Positive ventromedial columns were less prominent, and there was an increased expression throughout the spinal cord.
TS26: spinal cord ventral horn	Present	Regionally restricted	3B	Positive ventromedial columns were less prominent.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall		Present	Regionally restricted	3C	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
TS26: spinal cord thoracic region		Present	Regionally restricted	3C	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
TS26: spinal cord lumen	ependymal cell	Present		3C
TS26: spinal cord dorsal horn		Present		3C
TS26: spinal cord ventral horn		Present		3C

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall		Present	Regionally restricted	3D	The decreased prominence (62%, summarized in Table 1) of the ventromedial columns was partly due to the dispersion of these cells, compared to their clustered arrangement in the heterozygote.
TS26: spinal cord thoracic region		Present	Regionally restricted	3D	The decreased prominence (62%, summarized in Table 1) of the ventromedial columns was partly due to the dispersion of these cells, compared to their clustered arrangement in the heterozygote.
TS26: spinal cord dorsal horn	glial cell	Present	Regionally restricted	3D	Concentrated in the dorsal gray matter (summarized in Table 1).
TS26: spinal cord ventral horn		Present		3D	Numerous small positive cells were also found (summarized in Table 1).
TS26: spinal cord white matter		Present		3D	Numerous small positive cells were also found (summarized in Table 1).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall		Present	Regionally restricted	3E	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
TS26: spinal cord lumbar region		Present	Regionally restricted	3E	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
TS26: spinal cord lumen	ependymal cell	Present		3E
TS26: spinal cord ventral horn		Present	Regionally restricted	3E	Positive cells were concentrated in the most ventral portion.
TS26: spinal cord dorsal horn		Present	Regionally restricted	3E	Positive cells with small narrow nuclei were found in the dorsal spinal cord.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall	spinal cord motor neuron	Present	Regionally restricted	3F	Similar as in the heterozygous (summarized in Table 1). Expressed in the lateral motor column.
TS26: spinal cord lumbar region	spinal cord motor neuron	Present	Regionally restricted	3F	Similar as in the heterozygous (summarized in Table 1). Expressed in the lateral motor column.
TS26: spinal cord dorsal horn	glial cell	Present	Regionally restricted	3F	Concentrated in the dorsal gray matter, similar pattern as in the heterozygous, with an increased number and broader distribution (summarized in Table 1).
TS26: spinal cord ventral horn		Present		3F	Numerous small positive cells were also found.
TS26: spinal cord white matter		Present		3F	Numerous small positive cells were also found.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall	spinal cord motor neuron	Present	Regionally restricted	3G	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity). Only a subset of the ventromedial LacZ+ neurons were motor neurons and appeared to form a subset of the medial motor column.
TS26: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	3G	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity). Only a subset of the ventromedial LacZ+ neurons were motor neurons and appeared to form a subset of the medial motor column.
TS26: spinal cord ventral horn	glial cell	Present	Regionally restricted	3G	Only a few LacZ positive glial cells (small narrow nuclei) were found in the ventral spinal cord in the heterozygous mutant.
TS26: spinal cord dorsal horn	spinal cord ventral column interneuron	Present	Regionally restricted	3G	LacZ positive without acetylcholinesterase activity.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	3H	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity).
TS26: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	3H	Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity).
TS26: spinal cord lateral wall	glial cell	Present	Regionally restricted	3H	Many LacZ positive glial cells (small narrow nuclei) were found in the homozygous mutant.
TS26: spinal cord lateral wall	spinal cord ventral column interneuron	Present	Scattered	3H	LacZ positive without acetylcholinesterase activity. A 44% reduction of positive thoracic interneurons compared with the heterozygous (summarized in Table 1).
TS26: spinal cord ventral horn		Present		3H	Numerous small positive cells were also found.
TS26: spinal cord white matter		Present		3H	Numerous small positive cells were also found.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord cervical region	spinal cord motor neuron	Present	Single cells	4A	Very few positive cells were found in the medial motor column at this cervical level.
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Single cells	4A	Very few positive cells were found in the medial motor column at this cervical level.
TS21: spinal cord cervical region	spinal cord ventral column interneuron	Present		4A
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4A

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	4B	Expressed in a subset of cells (medial half) in the medial motor column.
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	4B	Expressed in a subset of cells (medial half) in the medial motor column.
TS21: spinal cord thoracic region	spinal cord ventral column interneuron	Present		4B
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4B

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	spinal cord motor neuron	Present	Regionally restricted	4C	Expressed in a subset of cells in the medial and lateral motor columns.
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	4C	Expressed in a subset of cells in the medial and lateral motor columns.
TS21: spinal cord lumbar region	spinal cord ventral column interneuron	Present		4C
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4C

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	spinal cord motor neuron	Present	Regionally restricted	4D	Expressed in a subset of cells in the medial and lateral motor columns.
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	4D	Expressed in a subset of cells in the medial and lateral motor columns.
TS21: spinal cord lumbar region	spinal cord ventral column interneuron	Present		4D
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4D

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord cervical region		Present	Single cells	4E	Very few positive cells were found in the medial motor column at this cervical level, similar as in the heterozygous.
TS21: spinal cord ventral horn		Present	Single cells	4E	Very few positive cells were found in the medial motor column at this cervical level, similar as in the heterozygous.
TS21: spinal cord cervical region	spinal cord ventral column interneuron	Present		4E
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	4F	Expressed in a subset of cells (medial half) in the medial motor column. A 49% decrease in thoracic positive cells compared with the heterozygous (summarized in Table 1).
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	4F	Expressed in a subset of cells (medial half) in the medial motor column. A 49% decrease in thoracic positive cells compared with the heterozygous (summarized in Table 1).
TS21: spinal cord thoracic region	spinal cord ventral column interneuron	Present	Regionally restricted	4F	A 42% increase in the thoracic positive interneuron population compared with the heterozygous (summarized in Table 1).
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present	Regionally restricted	4F	A 42% increase in the thoracic positive interneuron population compared with the heterozygous (summarized in Table 1).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	spinal cord motor neuron	Present	Regionally restricted	4G	Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted	4G	Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
TS21: spinal cord lumbar region	spinal cord ventral column interneuron	Present		4G	Increased by 93% compared to the heterozygous (summarized in Table 1).
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4G	Increased by 93% compared to the heterozygous (summarized in Table 1).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord lumbar region		Present	Regionally restricted	4H	Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
TS21: spinal cord ventral horn		Present	Regionally restricted	4H	Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
TS21: spinal cord lumbar region	spinal cord ventral column interneuron	Present		4H	Increased by 93% compared to the heterozygous (summarized in Table 1).
TS21: spinal cord ventral horn	spinal cord ventral column interneuron	Present		4H	Increased by 93% compared to the heterozygous (summarized in Table 1).

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present
TS22: inferior colliculus	Present
TS22: superior colliculus	Present

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall	glial cell	Present	Regionally restricted		Coexpressed with Gfap in a subset of cells with small narrow nuclei. LacZ positive cells in the medial and lateral ventral columns did not express glial marker Gfap.
TS26: basal ganglia		Absent
TS26: cerebral cortex		Absent

Structure	Level	Pattern	Image	Note
TS26: basal ganglia	Absent
TS26: cerebral cortex	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord lateral wall	glial cell	Present	Regionally restricted		Coexpressed with Nes in a subset of cells with small narrow nuclei. LacZ positive cells in the medial and lateral ventral columns did not express glial marker Nes.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: future spinal cord	spinal cord motor neuron	Present			Positive newly generated motor neurons were seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: future spinal cord	spinal cord motor neuron	Present			Positive newly generated motor neurons were seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	spinal cord ventral column interneuron	Present			Positive newly generated interneurons were seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord ventral horn	spinal cord motor neuron	Present	Regionally restricted		Expressed in a subset of cells in the lateral motor column. Very few positive cells were found in the medial motor column at this sacral level.