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Gene Expression Data
Assay Details
Assay
Reference: J:40032 Dou C, et al., TWH regulates the development of subsets of spinal cord neurons. Neuron. 1997 Apr;18(4):539-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7490072
Gene symbol: Isl2
Gene name: insulin related protein 2 (islet 2)
Antibody: anti-C-Isl-1 protein (K5)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 4A: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Cervical level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord cervical region spinal cord motor neuron Present Regionally restricted 4A Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4A Expressed in medial and lateral motor columns.
Specimen 4B: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Thoracic level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B Expressed in medial motor column.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4B Expressed in medial motor column.
Specimen 4C: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Upper lumbar level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4C Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4C Expressed in medial and lateral motor columns.
Specimen 4D: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Mid lumbar level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Level Pattern Image Note
TS21: spinal cord lumbar region Present Regionally restricted 4D Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn Present Regionally restricted 4D Expressed in medial and lateral motor columns.
Specimen 4E: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Cervical level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord cervical region spinal cord motor neuron Present Regionally restricted 4E Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4E Expressed in medial and lateral motor columns.
Specimen 4F: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Thoracic level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4F Expressed in medial motor column.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4F Expressed in medial motor column.
Specimen 4G: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Upper lumbar level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4G Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4G Expressed in medial and lateral motor columns.
Specimen 4H: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Mid lumbar level. Double labeled: brown - Isl2 and Isl1; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4H Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4H Expressed in medial and lateral motor columns.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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