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Gene Expression Data
Assay Details
Assay
Reference: J:213958 Lang RA, et al., p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development. 2014 Aug;141(16):3177-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7486718
Gene symbol: Ctnnb1
Gene name: catenin beta 1
Antibody: anti-beta-catenin (H-102, sc-7199)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4A Stage I flox/flox: embryonic day 10.0; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS16: lens pit Present 4A-1-I The first stage was defined as a lens placode with the first hint of inward bending.
Specimen 4A Stage II flox/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-1-II During the second stage, the lens cells elongated and the curvature of the lens placode extended to a shallow arc.
Specimen 4A Stage III flox/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-1-III During the third stage, the lens placode extended to a semicircular shape.
Specimen 4A Stage IV flox/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-1-IV An omega-shaped lens pit formed in the fourth stage due to the formation of hinge points.
Specimen 4A Stage V flox/flox: embryonic day 10.5-10.75; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-1-V The process of lens pit closure occured at stage V.
Specimen 4A E15.5 flox/flox: embryonic day 15.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS23: lens Present 4A-1-E15.5 The lens vesicle and the future cornea were normally separated.
Specimen 4A Stage I cKO: embryonic day 10.0; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: lens pit Present 4A-2-I Similar as in the control.
Specimen 4A Stage II cKO: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-2-II Similar as in the control.
Specimen 4A Stage III cKO: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-2-III Earliest sign of malformation: the lens pit had relatively straight epithelia without curvature at the lateral margins.
Specimen 4A Stage IV cKO: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-2-IV The lens pits lacked hinge points.
Specimen 4A Stage V cKO: embryonic day 10.5-10.75; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-2-V The lens pits lacked hinge points.
Specimen 4A E15.5 cKO: embryonic day 15.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lens Present 4A-2-E15.5 The lens pits pinched off, but the gap that is normally present between the lens vesicle and ocular mesenchyme was absent.
Specimen 4A Stage I Gt/Gt: embryonic day 10.0; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS16: lens pit Present 4A-3-I Similar as in the control.
Specimen 4A Stage II Gt/Gt: embryonic day 10.5; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-3-II Lateral lens pit epithelia started to appear quantitatively less curvature.
Specimen 4A Stage III Gt/Gt: embryonic day 10.5; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-3-III Lens pit had relatively straight epithelia without curvature at the lateral margins.
Specimen 4A Stage IV Gt/Gt: embryonic day 10.5; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-3-IV The lens pits lacked hinge points.
Specimen 4A Stage V Gt/Gt: embryonic day 10.5-10.75; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS17: lens pit Present 4A-3-V The lens pits lacked hinge points.
Specimen 4A E15.5 Gt/Gt: embryonic day 15.5; Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor (more )
Structure Level Pattern Image Note
TS23: lens Present 4A-3-E15.5 The lens vesicle was reduced in size and remained connected to the future cornea.
Specimen 4A Stage I +/Gt;+/flox: embryonic day 10.0; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: lens placode Present 4A-4-I Similar as in the control.
Specimen 4A Stage II +/Gt;+/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-4-II Lateral lens pit epithelia started to appear quantitatively less curvature.
Specimen 4A Stage III +/Gt;+/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-4-III Lens pit had relatively straight epithelia without curvature at the lateral margins. The cells were significantly wider at the apex and shorter than those of any of the other three genotypes analyzed (see Fig. 4D, E).
Specimen 4A Stage IV +/Gt;+/flox: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-4-IV The lens pits lacked hinge points.
Specimen 4A Stage V +/Gt;+/flox: embryonic day 10.5-10.75; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 4A-4-V The lens pits lacked hinge points. Embryos sometimes did not have a lens pit (not shown).
Specimen 4A E15.5 +/Gt;+/flox: embryonic day 15.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lens Present 4A-4-E15.5 The lens vesicle remained connected to the future cornea.
Specimen S2A: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present S2A Similar as in the control.
Specimen S2B: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present S2B Similar as in the control.
Specimen S2C: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present S2C Similar as in the control.
Specimen S2D: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present S2D Similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory