Shroom3^{Gt(ROSA53)Sor}
Gene trapped Allele Detail

Summary

• Symbol: Shroom3^{Gt(ROSA53)Sor}
• Name: shroom family member 3; gene trap ROSA53, Philippe Soriano
• MGI ID: MGI:1889778
• Synonyms: shrm^Gtrosa53, shroom, Shroom3^{Gt(ROSA)53Sor}, Shroom^Gt
• Gene: Shroom3 Location: Chr5:92831294-93113177 bp, + strand Genetic Position: Chr5, 47.29 cM, cytoband E3
• Alliance: Shroom3^{Gt(ROSA53)Sor} page

Mutation origin

• Mutant Cell Line: ROSA53
• Germline Transmission: Earliest citation of germline transmission: J:58961
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Gene trapped (Modified isoform(s), Recombinase, Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSABetagalCre
• Shroom3^{Gt(ROSA53)Sor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Shroom3 (MGI:1351655)

• Mutation details: Gene beta-gal and cre recombinase containing trap insertion disrupts expression of one of two alternative splice forms. Insertion occurred 3' of the translation start site for the longer transcript, but 5' to the translational start site for the shorter transcript. (J:58691)
• Inheritance: Recessive
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  FHCRC-GT-ROSA53	CG465839 ; (MGI Seq Detail)	5' RACE	Chr5:92831293-93067634 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  160 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Shroom3 (mouse) Summary of all recombinase alleles driven by Shroom3. Comparative matrix view of recombinase activities Alliance mouse Shroom3 gene page

Expression

In Mice Carrying this Mutation:	175 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Shroom3 Mutation: 204 strains or lines available

References

• Original: J:58691 Hildebrand JD, et al., Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell. 1999 Nov 24;99(5):485-97
• All: 14 reference(s)