Antibody:	anti-Vangl2 (2G4; MABN750)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS23: epidermis basal layer	basal cell of epidermis	Present	Regionally restricted	3B' top , 3B' bottom	Expression was detected in the basal layer of the interfollicular epidermis enriched along anterior-posterior (A-P) junctions and depleted from M-L junctions.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: epidermis basal layer	basal cell of epidermis	Present	Regionally restricted	3D' top , 3D' bottom	Expression was more uniform around the periphery of basal cells compared to controls.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: epidermis basal layer	basal cell of epidermis	Present	Regionally restricted	3F' top , 3F' bottom	Asymmetry was mostly unaffected in the Celsr2-/- mutant. Staining localization appeared less sharply concentrated at junctions.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: epidermis basal layer	basal cell of epidermis	Present	Regionally restricted	3H' top , 3H' bottom	Staining asymmetries were more severely reduced in Celsr1-/-;Celsr2-/- double mutants compared to Celsr1-/- single mutants. However, there was not an appreciable reduction in membrane recruitment of Vangl2.