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Gene Expression Data
Assay Details
Assay
Reference: J:146199 Barrionuevo F, et al., Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009 Mar 15;327(2):301-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464311
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Fixation with 3.7% formaldehyde. Two Wt1 antibodies were described, but the one used in this assay was not detailed. Anti-WT1 (M3561) MGI:3038122 may have been used in this assay.
Results
Specimen 5Bc: embryonic day 15.5 (more )
Structure Cell Type Level Pattern Image Note
TS23: Sertoli cell Sertoli cell Present 5Bc
Specimen 5Bc inset: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: maturing glomerular tuft Present 5Bc inset
Specimen 5Bd: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present 5Bd
Specimen 5Bg: embryonic day 15.5; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS23: Sertoli cell Sertoli cell Weak 5Bg Expression in Sertoli cells reduced compared to control.
Specimen 5Bg inset: embryonic day 15.5; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: maturing glomerular tuft Present 5Bg inset
Specimen 5Bh: postnatal day 0; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present 5Bh Expression in Sertoli cells not reduced at P day 0.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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