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Gene Expression Data
Assay Details
Assay
Reference: J:259707 Ye B, et al., Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. Nat Commun. 2018 Mar 28;9(1):1261
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7446722
Gene symbol: Klf4
Gene name: Kruppel-like transcription factor 4 (gut)
Results
Specimen S4h 1-cell: embryonic day 0.5 (more )
Note: Double labeled: green - Klf4; red - GT335.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent S4h 1-cell Klf4/GT335 , S4h 1-cell Klf4 , S4h 1-cell Klf4/GT335/DAPI
Specimen S4h 4-8 cell: embryonic day 2.5 (more )
Note: Double labeled: green - Klf4; red - GT335.
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Present S4h 4-8 cell Klf4/GT335/DAPI , S4h 4-8 cell Klf4/GT335 , S4h 4-8 cell Klf4 Authors report polyglutamylation of Klf in nucleus of early embryos.
Specimen S4h 16-32 cell: embryonic day 3.0 (more )
Note: Double labeled: green - Klf4; red - GT335.
Structure Level Pattern Image Note
TS4: compacted morula Present S4h 16-32 cell Klf4/GT335 , S4h 16-32 cell Klf4 , S4h 16-32 cell Klf4/GT335/DAPI
Specimen 4h Ttll4+/+: embryonic day 2.5 (more )
Note: Double labeled: green - Klf4; red - GT335.
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Present 4h Ttll4+/+ Klf4/GT335 , 4h Ttll4+/+ Klf4/GT335/DAPI , 4h Ttll4+/+ Klf4
Specimen 4h Ttll4-/-: embryonic day 2.5; Ttll4em2Zfa/Ttll4em2Zfa (more )
Note: Double labeled: green - Klf4; red - GT335.
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Absent 4h Ttll4-/- Klf4/GT335 , 4h Ttll4-/- Klf4 , 4h Ttll4-/- Klf4/GT335/DAPI Authors report hypoglutamylation of Klf4 resulting in degradation in 4-8-cell stage embryos.
Specimen 6c Nanog/Klf4 WT: embryonic day 3.5 (more )
Note: Double labeled: green - Klf4; red - Nanog (assay MGI:7446728).
Structure Level Pattern Image Note
TS5: blastocyst Present 6c Nanog/Klf4 WT
Specimen 6c Nanog/Klf4 E381A: embryonic day 3.5; Klf4em1Zfa/Klf4em1Zfa (more )
Note: Double labeled: green - Klf4; red - Nanog (assay MGI:7446728).
Structure Level Pattern Image Note
TS5: blastocyst Weak 6c Nanog/Klf4 E381A Expression substantially reduced.
Specimen 6c Gata6/Klf4 WT: embryonic day 3.5 (more )
Note: Double labeled: green - Klf4; red - Gata6 (assay MGI:7446730).
Structure Level Pattern Image Note
TS5: blastocyst Present 6c Gata6/Klf4 WT
Specimen 6c Gata6/Klf4 E381A: embryonic day 3.5; Klf4em1Zfa/Klf4em1Zfa (more )
Note: Double labeled: green - Klf4; red - Gata6 (assay MGI:7446730).
Structure Level Pattern Image Note
TS5: blastocyst Weak 6c Gata6/Klf4 E381A Expression substantially reduced.
Specimen 6c Cdx2/Klf4 WT: embryonic day 3.5 (more )
Note: Double labeled: green - Klf4; red - Cdx2 (assay MGI:7446731).
Structure Level Pattern Image Note
TS5: blastocyst Present 6c Cdx2/Klf4 WT
Specimen 6c Cdx2/Klf4 E381A: embryonic day 3.5; Klf4em1Zfa/Klf4em1Zfa (more )
Note: Double labeled: green - Klf4; red - Cdx2 (assay MGI:7446731).
Structure Level Pattern Image Note
TS5: blastocyst Weak 6c Cdx2/Klf4 E381A Expression substantially reduced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory