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Gene Expression Data
Assay Details
Assay
Reference: J:259707 Ye B, et al., Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. Nat Commun. 2018 Mar 28;9(1):1261
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7446728
Gene symbol: Nanog
Gene name: Nanog homeobox
Results
Specimen 6c Nanog/Klf4 WT: embryonic day 3.5 (more )
Note: Double labeled: red - Nanog; green - Klf4 (assay MGI:7446722).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 6c Nanog/Klf4 WT Expression as marker for epiblast.
Specimen 6c Nanog/Klf4 E381A: embryonic day 3.5; Klf4em1Zfa/Klf4em1Zfa (more )
Note: Double labeled: red - Nanog; green - Klf4 (assay MGI:7446722).
Structure Level Pattern Image Note
TS5: inner cell mass Weak 6c Nanog/Klf4 E381A Expression substantially reduced.
Specimen 6d Nanog/Casp3 WT: embryonic day 3.5 (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 6d Nanog/Casp3 WT Expression as marker for epiblast.
Specimen 6d Nanog/Casp3 E381A: embryonic day 3.5; Klf4em1Zfa/Klf4em1Zfa (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Weak 6d Nanog/Casp3 E381A Expression substantially reduced.
Specimen S7c Nanog/Casp3 WT: embryonic day 3.5 (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Present Punctate S7c Nanog/Casp3 WT Expression as marker for epiblast.
Specimen S7c Nanog/Casp3 Ccp1-/-: embryonic day 3.5; Agtpbp1pcd-3J/Agtpbp1pcd-3J (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted S7c Nanog/Casp3 Ccp1-/- Expression as marker for epiblast.
Specimen S7c Nanog/Casp3 Ccp6-/-: embryonic day 3.5; Agbl4Tn(pb-Act-RFP)1.080115015-HRAZhu/Agbl4Tn(pb-Act-RFP)1.080115015-HRAZhu (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted S7c Nanog/Casp3 Ccp6-/- Expression as marker for epiblast.
Specimen S7c Nanog/Casp3 Ttll1-/-: embryonic day 3.5; Ttll1em1Zfa/Ttll1em1Zfa (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Ambiguous S7c Nanog/Casp3 Ttll1-/- Authors report disordered lineage marker expression.
Specimen S7c Nanog/Casp3 Ttll4-/-: embryonic day 3.5; Ttll4em2Zfa/Ttll4em2Zfa (more )
Note: Double labeled: red - Nanog; green - Casp3 (assay MGI:7446732).
Structure Level Pattern Image Note
TS5: inner cell mass Ambiguous S7c Nanog/Casp3 Ttll4-/- Authors report disordered lineage marker expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory