Ttll4^em2Zfa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ttll4^em2Zfa
• Name: tubulin tyrosine ligase-like family, member 4; endonuclease-mediated mutation 2, Zusen Fan
• MGI ID: MGI:6392148
• Gene: Ttll4 Location: Chr1:74700804-74740991 bp, + strand Genetic Position: Chr1, 38.54 cM
• Alliance: Ttll4^em2Zfa page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated an 8 bp deletion (ACCGCCGC) in exon 3 resulting in a frameshift mutation. Western blot analysis confirmed absence of protein in mouse embryonic fibroblasts. (J:259707)

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttll4 Mutation: 71 strains or lines available

References

• Original: J:259707 Ye B, et al., Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. Nat Commun. 2018 Mar 28;9(1):1261
• All: 1 reference(s)