Nkx2-1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:225740 Snowball J, et al., Endodermal Wnt signaling is required for tracheal cartilage formation. Dev Biol. 2015 Sep 1;405(1):56-70
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7407326
Gene symbol:	Nkx2-1
Gene name:	NK2 homeobox 1

Antibody:	anti-Nkx2.1
Detection system:	Secondary antibody

Results

Specimen 5A right control: embryonic day 11.5 (more

)
Note: Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS19: trachea epithelium	Present		5A right upper

Specimen 5A right cKO: embryonic day 11.5; Shh^{tm1(EGFP/cre)Cjt}/Shh⁺, Wls^tm1.1Lan/Wls^tm1.1Lan (more

)
Note: Conditional mutant. Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS19: trachea epithelium	Present		5A right lower

6C ©

Specimen 6C: embryonic day 14.5 (more

)
Note: Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6C

6D ©

Specimen 6D: embryonic day 14.5; Sox9^tm1Gsr/Sox9^tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more

)
Note: Conditional mutant. Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6D

6E ©

Specimen 6E: embryonic day 14.5 (more

)
Note: Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6E

6F ©

Specimen 6F: embryonic day 14.5; Sox9^tm1Gsr/Sox9^tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more

)
Note: Conditional mutant. Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6F

6G ©

Specimen 6G: embryonic day 14.5 (more

)
Note: Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6G

6H ©

Specimen 6H: embryonic day 14.5; Sox9^tm1Gsr/Sox9^tm1Gsr, Tg(Col2a1-cre)1Bhr/0 (more

)
Note: Conditional mutant. Triple labeled: purple - Nkx2-1; green - Sox9 (assay MGI:7407287); red - Acta2 (assay MGI:7388675).

Structure	Level	Pattern	Image	Note
TS22: trachea epithelium	Present		6H

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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