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Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388341
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-TTF1 (WRAB-1231)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 7A control: embryonic day 14.5 (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 7A lower control
Specimen 7A -/-: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 7A lower -/- Similar as in the control.
Specimen 9C control: embryonic day 12.5 (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS20: trachea epithelium Present 9C control lower , 9C control upper
Specimen 9C mut: embryonic day 12.5; Ctnnb1tm1Mmt/Ctnnb1+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS20: trachea epithelium Present 9C cKO lower , 9C cKO upper Similar as in the control.
Specimen 9D Ror2 f/f: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D left upper , 9D left lower
Specimen 9D Ror2 cKO: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D middle lower , 9D middle upper Tracheal stenosis was seen.
Specimen 9D Notum 150/150: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D right lower , 9D right upper Tracheal stenosis was seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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