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Gene Expression Data
Assay Details
Assay
Reference: J:329930 Guertin TM, et al., Deciphering the role of retinoic acid in hepatic patterning and induction in the mouse. Dev Biol. 2022 Nov;491:31-42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7378252
Gene symbol: Hnf4a
Gene name: hepatic nuclear factor 4, alpha
Results
Specimen 3C Anterior: embryonic day 9.5 (more )
Note: Anterior liver bud section. Control was either Rdh10+/+ or Rdh10trex/+. 27 somites. Double labeled: red - Hnf4a; green - Prox1 (assay MGI:7378253).
Structure Cell Type Level Pattern Image Note
TS15: hepatic primordium hepatoblast Present Uniform 3C Anterior Merge , 3C Anterior Hnf4a
Specimen 3C Middle: embryonic day 9.5 (more )
Note: Middle liver bud section. Control was either Rdh10+/+ or Rdh10trex/+. 27 somites. Double labeled: red - Hnf4a; green - Prox1 (assay MGI:7378253).
Structure Cell Type Level Pattern Image Note
TS15: hepatic primordium hepatoblast Present Uniform 3C Middle Hnf4a , 3C Middle Merge
Specimen 3D Anterior: embryonic day 9.5; Rdh10trex/Rdh10trex (more )
Note: Anterior liver bud section. 23-24 somites. Double labeled: red - Hnf4a; green - Prox1 (assay MGI:7378253).
Structure Cell Type Level Pattern Image Note
TS15: hepatic primordium hepatoblast Present Regionally restricted 3D Anterior Merge , 3D Anterior Hnf4a Expression is significantly reduced in the dorsal half of the anterior liver bud.
Specimen 3D Middle: embryonic day 9.5; Rdh10trex/Rdh10trex (more )
Note: Middle liver bud section. 23-24 somites. Double labeled: red - Hnf4a; green - Prox1 (assay MGI:7378253).
Structure Cell Type Level Pattern Image Note
TS15: hepatic primordium hepatoblast Present Uniform 3D Middle Hnf4a , 3D Middle Merge
Specimen 5A': embryonic day 10.5 (more )
Note: Control was either Rdh10+/+ or Rdh10trex/+. Triple labeled: red - Hnf4a; green - Gata4 (assay MGI:7378255); white - Isl1 (assay MGI:7378256).
Structure Cell Type Level Pattern Image Note
TS17: hepatic primordium hepatoblast Present Regionally restricted 5A' HNF4a-positive hepatoblasts are distributed throughout the GATA4-positive hepatic mesoderm of both the rostral lobe (blue asterisk) and the smaller caudal lobes (yellow asterisks).
Specimen 5B': embryonic day 10.5; Rdh10trex/Rdh10trex (more )
Note: Triple labeled: red - Hnf4a; green - Gata4 (assay MGI:7378255); white - Isl1 (assay MGI:7378256).
Structure Cell Type Level Pattern Image Note
TS17: hepatic primordium hepatoblast Present 5B' Defects in the caudal lobe were noted.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory