Pcdh19 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:255320 Pederick DT, et al., Abnormal Cell Sorting Underlies the Unique X-Linked Inheritance of PCDH19 Epilepsy. Neuron. 2018 Jan 3;97(1):59-66.e5
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7329910
Gene symbol:	Pcdh19
Gene name:	protocadherin 19

Reporter:	HA
Antibody:	anti-HA tag (C29F4) (#3724)
Detection system:	Secondary antibody coupled to Texas Red

Results

Specimen 2D HA-FLAG/beta-Geo: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present	Regionally restricted	2D HA-FLAG/beta-Geo X-GAL , 2D HA-FLAG/beta-Geo	Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
TS22: cerebral cortex ventricular layer	Present	Regionally restricted	2D HA-FLAG/beta-Geo X-GAL , 2D HA-FLAG/beta-Geo	Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
TS22: cortical plate	Present	Regionally restricted	2D HA-FLAG/beta-Geo X-GAL , 2D HA-FLAG/beta-Geo	Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.

Specimen 2D HA-FLAG/+: embryonic day 14.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present	Regionally restricted	2D HA-FLAG/+ , 2D HA-FLAG/+ X-GAL	There were small interspersed patches of HA-positive and HA-negative staining along the ventricle and overlying neural progenitors in the ventricular zone.
TS22: cerebral cortex intermediate zone	Present		2D HA-FLAG/+ , 2D HA-FLAG/+ X-GAL
TS22: cortical plate	Present		2D HA-FLAG/+ , 2D HA-FLAG/+ X-GAL

Specimen 2G HA-FLAG/+: postnatal day 7; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2G HA-FLAG/+

Specimen 2G HA-FLAG/beta-Geo: postnatal day 7; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2G HA-FLAG/beta-Geo	There were small interspersed patches of HA-positive and HA-negative staining.

Specimen S2D: embryonic day 14.5; Pcdh19^em1Pqt/Y (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		S2D

Specimen S2F left: postnatal day 7; Pcdh19^em1Pqt/Y (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		S2F left
TS28: cortical layer V	Present		S2F left

Specimen S2F right panel top left: postnatal day 7; Pcdh19^em1Pqt/Y (more

)
Note: Double labeled: green - Pcdh19; magenta - Bcl11b (assay MGI:7329937).

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		S2F right panel top right , S2F right panel top left
TS28: cortical layer V	Present		S2F right panel top right , S2F right panel top left

Specimen S2H 13.5: embryonic day 13.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		S2H E13.5

Specimen S2H 14.5 left: embryonic day 14.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		S2H E14.5 left

Specimen S2H 14.5 middle: embryonic day 14.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		S2H E14.5 middle

Specimen S2H 14.5 right: embryonic day 14.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		S2H E14.5 right

Specimen S2H 15.5: embryonic day 15.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		S2H E15.5

Specimen S2I 13.5: embryonic day 13.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	S2I E13.5	There are areas of segregated positive and negative cells.
TS21: ganglionic eminence	Present	Regionally restricted	S2I E13.5	There are areas of segregated positive and negative cells.

Specimen S2I 14.5 left: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2I E14.5 left	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2I E14.5 left	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2I E14.5 left	There are areas of segregated positive and negative cells.

Specimen S2I 14.5 middle: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2I E14.5 middle	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2I E14.5 middle	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2I E14.5 middle	There are areas of segregated positive and negative cells.

Specimen S2I 14.5 right: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2I E14.5 right	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2I E14.5 right	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2I E14.5 right	There are areas of segregated positive and negative cells.

Specimen S2I 15.5: embryonic day 15.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	S2I E15.5	There are areas of segregated positive and negative cells.
TS23: ganglionic eminence	Present	Regionally restricted	S2I E15.5	There are areas of segregated positive and negative cells.
TS23: hippocampus	Present	Regionally restricted	S2I E15.5	There are areas of segregated positive and negative cells.

Specimen S2J 13.5: embryonic day 13.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	S2J E13.5	There are areas of segregated positive and negative cells.
TS21: ganglionic eminence	Present	Regionally restricted	S2J E13.5	There are areas of segregated positive and negative cells.

Specimen S2J 14.5 left: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2J E14.5 left	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2J E14.5 left	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2J E14.5 left	There are areas of segregated positive and negative cells.

Specimen S2J 14.5 middle: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2J E14.5 middle	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2J E14.5 middle	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2J E14.5 middle	There are areas of segregated positive and negative cells.

Specimen S2J 14.5 right: embryonic day 14.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S2J E14.5 right	There are areas of segregated positive and negative cells.
TS22: ganglionic eminence	Present	Regionally restricted	S2J E14.5 right	There are areas of segregated positive and negative cells.
TS22: hippocampus	Present	Regionally restricted	S2J E14.5 right	There are areas of segregated positive and negative cells.

Specimen S2J 15.5: embryonic day 15.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	S2J E15.5	There are areas of segregated positive and negative cells.
TS23: ganglionic eminence	Present	Regionally restricted	S2J E15.5	There are areas of segregated positive and negative cells.
TS23: hippocampus	Present	Regionally restricted	S2J E15.5	There are areas of segregated positive and negative cells.

Specimen S3A left: embryonic day 10.5; Pcdh19^em1Pqt/Y (more

)
Note: Double labeled with phalloidin (magenta).

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present		S3A left top , S3A left bottom	Expression is at all cell-cell boundaries.

Specimen S3A middle: embryonic day 10.5; Pcdh19^em1Pqt/Pcdh19⁺ (more

)
Note: Double labeled with phalloidin (magenta).

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present	Regionally restricted	S3A middle top , S3A middle bottom	Expression is at boundaries between HA-positive and HA-negative cells.

Specimen S3A right: embryonic day 10.5; Pcdh19^tm1Tac/Pcdh19^em1Pqt (more

)
Note: Double labeled with phalloidin (magenta).

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present	Regionally restricted	S3A right top , S3A right bottom	Expression is absent at boundaries between HA-positive and HA-negative cells.

Specimen S3C: embryonic day 8.5; Pcdh19^em1Pqt/Y (more