Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:255320
Pederick DT, et al., Abnormal Cell Sorting Underlies the Unique X-Linked Inheritance of PCDH19 Epilepsy. Neuron. 2018 Jan 3;97(1):59-66.e5
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:7329912
|
|
Gene symbol:
|
Pcdh19
|
|
Gene name:
|
protocadherin 19
|
|
Reporter:
|
Beta-geo
|
|
|
Direct Detection
|
|
Assay notes:
|
Images were false colored.
|
|
|
Results
|
Specimen
2D HA-FLAG/beta-Geo X-GAL:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more  )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
2D HA-FLAG/beta-Geo X-GAL:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex intermediate zone |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
| TS22: cerebral cortex ventricular layer |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
| TS22: cortical plate |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
Specimen
S2J 13.5:
embryonic day 13.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
S2J 13.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
Regionally restricted |
S2J E13.5
|
There are areas of segregated positive and negative cells.
|
| TS21: ganglionic eminence |
Present |
Regionally restricted |
S2J E13.5
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 left:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
S2J 14.5 left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 middle:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
S2J 14.5 middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 right:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
S2J 14.5 right:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 15.5:
embryonic day 15.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
Specimen
S2J 15.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: magenta - Pcdh19 beta-Geo reporter; green - Pcdh19 HA reporter (assay MGI:7329910).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: ganglionic eminence |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: hippocampus |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
|
Analysis Tools