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Gene Expression Data
Assay Details
Assay
Reference: J:324709 Wang Y, et al., The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development. Development. 2022 May 1;149(9):dev200340
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7327399
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-Lef1 (#2230)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen S4A WT FL: embryonic day 11.0 (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: forelimb bud Present S4A left middle , S4A left lower
Specimen S4A WT HL: embryonic day 11.0 (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: hindlimb bud Present S4A right lower , S4A right middle
Specimen S4B Wnt7a-/-7b+/- FL-1: embryonic day 11.0; Wnt7atm1Amc/Wnt7atm1Amc, Wnt7btm1Parr/Wnt7b+ (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: forelimb bud Present S4B FL left lower , S4B FL left middle
Specimen S4B Wnt7a-/-7b+/- FL-2: embryonic day 11.0; Wnt7atm1Amc/Wnt7atm1Amc, Wnt7btm1Parr/Wnt7b+ (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: forelimb bud Present S4B FL right lower , S4B FL right middle
Specimen S4B Wnt7a-/-7b+/- HL-1: embryonic day 11.0; Wnt7atm1Amc/Wnt7atm1Amc, Wnt7btm1Parr/Wnt7b+ (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: hindlimb bud Present S4B HL left lower , S4B HL left middle
Specimen S4B Wnt7a-/-7b+/- HL-2: embryonic day 11.0; Wnt7atm1Amc/Wnt7atm1Amc, Wnt7btm1Parr/Wnt7b+ (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: hindlimb bud Present S4B HL right lower , S4B HL right middle
Specimen S4C Reck FL-1: embryonic day 11.0; Recktm1.2Noda/Reckem2Nat (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: forelimb bud Present S4C FL left lower , S4C FL left middle
Specimen S4C Reck FL-2: embryonic day 11.0; Recktm1.2Noda/Reckem2Nat (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: forelimb bud Present S4C FL right lower , S4C FL right middle
Specimen S4C Reck HL-1: embryonic day 11.0; Recktm1.2Noda/Reckem2Nat (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: hindlimb bud Present S4C HL left lower , S4C HL left middle
Specimen S4C Reck HL-2: embryonic day 11.0; Recktm1.2Noda/Reckem2Nat (more )
Note: Imaged from the dorsal side. Double labeled: red - Lef1; green - Lmx1b (assay MGI:7327349).
Structure Level Pattern Image Note
TS18: hindlimb bud Present S4C HL right middle , S4C HL right lower
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory