Reck^em2Nat
Endonuclease-mediated Allele Detail

Summary

• Symbol: Reck^em2Nat
• Name: reversion-inducing-cysteine-rich protein with kazal motifs; endonuclease-mediated mutation 2, Jeremy Nathans
• MGI ID: MGI:6283835
• Synonyms: Reck^P256A,W261A
• Gene: Reck Location: Chr4:43875530-43944806 bp, + strand Genetic Position: Chr4, 23.05 cM
• Alliance: Reck^em2Nat page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing is used to insert alanine substitution mutations P256A (nucleotide change: CCT to GCA) and W261A (nucleotide change: TGG to GCC). The mutations eliminate Wnt7a signaling by the Reck gene, but do not affect protein folding or trafficking to the plasma membrane. (J:277288)

Expression

In Mice Carrying this Mutation:

21 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Reck Mutation: 46 strains or lines available

References

• Original: J:277288 Cho C, et al., Molecular determinants in Frizzled, Reck, and Wnt7a for ligand-specific signaling in neurovascular development. Elife. 2019 Jun 21;8:e47300
• All: 3 reference(s)