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Gene Expression Data
Assay Details
Assay
Reference: J:151715 Bradford ST, et al., A cell-autonomous role for WT1 in regulating Sry in vivo. Hum Mol Genet. 2009 Sep 15;18(18):3429-38
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7286366
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67 (TEC-3; M7249)
Detection system: Secondary antibody
Results
Specimen 1 Wild Type: embryonic day 11.5 (more )
Note: Triple labeled: white - Mki67; green - Sry (assay MGI:7286363); red - OCT4 (Pou5f1 assay MGI:7286365).
Structure Level Pattern Image Note
TS19: urogenital ridge Present Regionally restricted 1_Wild Type Merge , 1_Wild Type Ki67 Expression in proliferating cells.
Specimen 1 WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Triple labeled: white - Mki67; green - Sry (assay MGI:7286363); red - OCT4 (Pou5f1 assay MGI:7286365).
Structure Level Pattern Image Note
TS19: urogenital ridge Present Regionally restricted 1_WT1(+KTS)-/- Ki67 , 1_WT1(+KTS)-/- Merge Expression in proliferating cells.
Specimen 3A Wild Type: embryonic day 11.5 (more )
Note: Double labeled: red - Mki67; green - Sry (assay MGI:7286363).
Structure Level Pattern Image Note
TS19: coelomic epithelium of gonad Present 3A Wild Type
Specimen 3A WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: red - Mki67; green - Sry (assay MGI:7286363).
Structure Level Pattern Image Note
TS19: coelomic epithelium of gonad Weak 3A WT1(+KTS)-/- Number of expressing cells at or near coelomic epithelium significantly reduced compared with wild type.
Specimen 4A Wild Type: embryonic day 11.5 (more )
Note: Double labeled: red - Mki67; green - SF1 (Nr5a1 assay MGI:7286367).
Structure Level Pattern Image Note
TS19: gonad Present 4A Wild Type
Specimen 4A WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: red - Mki67; green - SF1 (Nr5a1 assay MGI:7286367).
Structure Level Pattern Image Note
TS19: gonad Present 4A WT1(+KTS)-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory