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Gene Expression Data
Assay Details
Assay
Reference: J:151715 Bradford ST, et al., A cell-autonomous role for WT1 in regulating Sry in vivo. Hum Mol Genet. 2009 Sep 15;18(18):3429-38
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7286365
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-OCT3/4
Detection system: Secondary antibody
Results
Specimen 1 Wild Type: embryonic day 11.5 (more )
Note: Triple labeled: red - OCT4 (Pou5f1); green - Sry (assay MGI:7286363); white - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: primordial germ cell Present 1_Wild Type OCT4 , 1_Wild Type Merge
TS19: urogenital ridge Present 1_Wild Type OCT4 , 1_Wild Type Merge
Specimen 1 WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Triple labeled: red - OCT4 (Pou5f1); green - Sry (assay MGI:7286363); white - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: primordial germ cell Present 1_WT1(+KTS)-/- Merge , 1_WT1(+KTS)-/- OCT4
TS19: urogenital ridge Present 1_WT1(+KTS)-/- Merge , 1_WT1(+KTS)-/- OCT4
Specimen 2A Wild Type: embryonic day 11.5 (more )
Note: Double labeled: red - OCT4 (Pou5f1); green - Sry (assay MGI:7286363).
Structure Level Pattern Image Note
TS19: gonad Present 2A Wild Type Merge , 2A Wild Type OCT4
TS19: primordial germ cell of gonad Present 2A Wild Type Merge , 2A Wild Type OCT4
Specimen 2A WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: red - OCT4 (Pou5f1); green - Sry (assay MGI:7286363).
Structure Level Pattern Image Note
TS19: gonad Present 2A WT1(+KTS)-/- OCT4 , 2A WT1(+KTS)-/- Merge
TS19: primordial germ cell of gonad Present 2A WT1(+KTS)-/- OCT4 , 2A WT1(+KTS)-/- Merge
Specimen 6C Wild Type: embryonic day 11.5 (more )
Note: Double labeled: red - OCT4 (Pou5f1); green - Sox9 (assay MGI:7286368).
Structure Level Pattern Image Note
TS19: gonad Present 6C Wild Type Merge , 6C Wild Type OCT4
TS19: primordial germ cell of gonad Present 6C Wild Type Merge , 6C Wild Type OCT4
Specimen 6C WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: red - OCT4 (Pou5f1); green - Sox9 (assay MGI:7286368).
Structure Level Pattern Image Note
TS19: gonad Present 6C WT1(+KTS)-/- Merge , 6C WT1(+KTS)-/- OCT4
TS19: primordial germ cell of gonad Present 6C WT1(+KTS)-/- Merge , 6C WT1(+KTS)-/- OCT4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory