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Gene Expression Data
Assay Details
Assay
Reference: J:151715 Bradford ST, et al., A cell-autonomous role for WT1 in regulating Sry in vivo. Hum Mol Genet. 2009 Sep 15;18(18):3429-38
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7286363
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Antibody: Anti-Sry
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1 Wild Type: embryonic day 11.5 (more )
Note: Triple labeled: green - Sry; red - OCT4 (Pou5f1 assay MGI:7286365); white - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: urogenital ridge Present Regionally restricted 1_Wild Type SRY , 1_Wild Type Merge Nuclear expression in pre-Sertoli cells. No overlap in expression with Oct4 (Pou5f1) or Mki67.
Specimen 1 WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Triple labeled: green - Sry; red - OCT4 (Pou5f1 assay MGI:7286365); white - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: urogenital ridge Present 1_WT1(+KTS)-/- Merge , 1_WT1(+KTS)-/- SRY
Specimen 2A Wild Type: embryonic day 11.5 (more )
Note: Double labeled: green - Sry; red - OCT4 (Pou5f1 assay MGI:7286365).
Structure Level Pattern Image Note
TS19: gonad Present 2A Wild Type Merge , 2A Wild Type SRY
Specimen 2A WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: green - Sry; red - OCT4 (Pou5f1 assay MGI:7286365).
Structure Level Pattern Image Note
TS19: gonad Weak 2A WT1(+KTS)-/- SRY , 2A WT1(+KTS)-/- Merge Lower levels of expression compared to wild type.
Specimen 3A Wild Type: embryonic day 11.5 (more )
Note: Double labeled: green - Sry; red - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: coelomic epithelium of gonad Present 3A Wild Type
Specimen 3A WT1(+KTS)-/-: embryonic day 11.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: green - Sry; red - Mki67 (assay MGI:7286366).
Structure Level Pattern Image Note
TS19: coelomic epithelium of gonad Weak 3A WT1(+KTS)-/- Clear reduction in number of expressing cells compared with wild-type
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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