Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72517
Hajihosseini MK, et al., A splicing switch and gain-of-function mutation in FgfR2-IIIc hemizygotes causes Apert/Pfeiffer-syndrome-like phenotypes. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3855-60
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7284945
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Gene symbol:
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Fgfr2
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Gene name:
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fibroblast growth factor receptor 2
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Probe:
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FgfR2IIIb probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5E +/+:
embryonic day 18.5
(more  )
Specimen
5E +/+:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: suture |
Trace |
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5E +/+
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Staining was barely detectable in calvarial sutures in the wild type mice.
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Specimen
5E +/delta:
embryonic day 18.5; Fgfr2tm2.3Dsn/Fgfr2 +
(more )
Specimen
5E +/delta:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * FVB/N
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Mutant Allele(s):
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Fgfr2tm2.3Dsn/Fgfr2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: suture |
Present |
Regionally restricted |
5E +/delta
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Calvarial sutures showed elevated expression of the Fgfr2-IIIb isoform in the hemizygote.
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Specimen
5I:
embryonic day 18.0
(more )
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: sternebra |
Strong |
Regionally restricted |
5I
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Strong expression was detected in the periosteal region of each sternebrae.
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Specimen
5J:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * FVB/N
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Mutant Allele(s):
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Fgfr2tm2.3Dsn/Fgfr2+
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: sternebra |
Strong |
Regionally restricted |
5J
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Strong expression was detected on the periosteal region of each sternebrae. Expression was also detected in the hemi-sternebrae medial rims in hemizygotes.
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Analysis Tools
