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Gene Expression Data
Assay Details
Assay
Reference: J:306246 He M, et al., Chloride channels regulate differentiation and barrier functions of the mammalian airway. Elife. 2020 Apr 14;9:e53085
Assay type: RNA in situ
MGI Accession ID: MGI:6781966
Gene symbol: Foxj1
Gene name: forkhead box J1
Probe: Mm-Foxj1 (317091)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 3 S1A early E16 - left: embryonic day 16.0 (more )
Note: Triple labeled: magenta - Foxj1; orange - Shisa8 (assay MGI:6781968); teal - Foxn4 (assay MGI:6781969).
Structure Level Pattern Image Note
TS24: trachea epithelium Present Regionally restricted 3 S1A early 16 left Foxj1 , 3 S1A early 16 left merge Expression is seen in immature ciliated cells.
Specimen 3 S1A early E16 - right: embryonic day 16.0 (more )
Note: Triple labeled: magenta - Foxj1; orange - Mcidas (assay MGI:6781985); teal - Foxn4 (assay MGI:6781969).
Structure Level Pattern Image Note
TS24: trachea epithelium Present Regionally restricted 3 S1A early 16 right merge , 3 S1A early 16 right Foxj1 Expression is seen in immature ciliated cells.
Specimen 3 S1A late E16: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Foxj1; orange - Shisa8 (assay MGI:6781968); teal - Foxn4 (assay MGI:6781969).
Structure Level Pattern Image Note
TS24: trachea epithelium Present Regionally restricted 3 S1A late 16 Foxj1 , 3 S1A late 16 merge Expression is seen in immature ciliated cells.
Specimen 4B: postnatal day 3 (more )
Note: Triple labeled: magenta - Foxj1; teal - Gp2 (assay MGI:6781971); orange - Prr18 (assay MGI:6781972).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 4B Foxj1/Prr18 , 4B Gp2/Foxj1 , 4B merge Although this is considered a ciliated cell marker, expression is also seen in "hybrid" cells that express secretory cell markers.
Specimen 4 S1A wt lower: postnatal newborn (more )
Note: Double labeled: red - Foxj1; green - Gp2 (assay MGI:6781971).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 4 S1A lower left Expression is seen in ciliated cells and in "hybrid" cells that express both ciliated and secretory cell markers.
Specimen 4 S1A Pofut-/- lower: postnatal newborn; Pofut1tm1Pst/Pofut1tm1Pst (more )
Note: Double labeled: red - Foxj1; green - Gp2 (assay MGI:6781971).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 4 S1A lower right Expression is seen in ciliated cells and in "hybrid" cells that express both ciliated and secretory cell markers. These mutants have abundant numbers of ciliated cells but lack secretory cells.
Specimen 7D wt: embryonic day 16.0 (more )
Note: Triple labeled: teal - Foxj1; magenta - Sostdc1 (assay MGI:6781983); yellow - Ccl20 (assay MGI:6781984).
Structure Level Pattern Image Note
TS24: trachea epithelium Present Regionally restricted 7D wt merge Expression is seen in immature ciliated cells.
Specimen 7D Ano1-/-: embryonic day 16.0; Ano1tm1Bdh/Ano1tm1Bdh (more )
Note: Triple labeled: teal - Foxj1; magenta - Sostdc1 (assay MGI:6781983); yellow - Ccl20 (assay MGI:6781984).
Structure Level Pattern Image Note
TS24: trachea epithelium Present Regionally restricted 7D Ano1-/- merge Expression is seen in immature ciliated cells; their number is comparable to those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory