Sycp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:253477 Bailey AS, et al., The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife. 2017 Oct 31;6:e26116
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6724544
Gene symbol:	Sycp3
Gene name:	synaptonemal complex protein 3

Antibody:	anti-SCP3 [Cor 10G11/7] (ab97672)
Detection system:	Alexa Fluor

Results

1D ©

Specimen 1D: postnatal adult (more

)
Note: Triple labeled: red - Sycp3; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present	Regionally restricted	1D

1E ©

Specimen 1E: postnatal adult (more

)
Note: Triple labeled: red - Sycp3; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1E

4E wild type (merge) ©

4E wild type SYCP3 ©

Specimen 4E wild type: postnatal day 14 (more

)
Note: Triple labeled: green - Sycp3; red - Rec8 (assay MGI:6724550); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4E wild type SYCP3 , 4E wild type (merge)

4E Ythdc2-/- (merge) ©

4E Ythdc2-/- SYCP3 ©

Specimen 4E Ythdc2-/-: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: green - Sycp3; red - Rec8 (assay MGI:6724550); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4E Ythdc2-/- (merge) , 4E Ythdc2-/- SYCP3

4F wild type (merge) ©

4F wild type SYCP3 ©

Specimen 4F wild type: postnatal day 14 (more

)
Note: Double labeled: green - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4F wild type SYCP3 , 4F wild type (merge)

4F Ythdc2-/- (merge) ©

4F Ythdc2-/- SYCP3 ©

Specimen 4F Ythdc2-/-: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4F Ythdc2-/- (merge) , 4F Ythdc2-/- SYCP3

4 S1A wild type (merge) ©

4 S1A wild type SYCP3 ©

Specimen 4-S1A wild type: postnatal day 14 (more

)
Note: Triple labeled: green - Sycp3; red - gH2AX (H2ax assay MGI:6724560); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4 S1A wild type (merge) , 4 S1A wild type SYCP3

4 S1A Ythdc2-/- (merge) ©

4 S1A Ythdc2-/- SYCP3 ©

Specimen 4-S1A Ythdc2-/-: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: green - Sycp3; red - gH2AX (H2ax assay MGI:6724560); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4 S1A Ythdc2-/- SYCP3 , 4 S1A Ythdc2-/- (merge)

4 S1B wild type (merge) ©

4 S1B wild type SYCP3 ©

Specimen 4-S1B wild type: postnatal day 14 (more

)
Note: Triple labeled: green - Sycp3; red - gH2AX (H2ax assay MGI:6724560); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4 S1B wild type SYCP3 , 4 S1B wild type (merge)

4 S1B Ythdc2-/- (merge) ©

4 S1B Ythdc2-/- SYCP3 ©

Specimen 4-S1B Ythdc2-/-: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: green - Sycp3; red - gH2AX (H2ax assay MGI:6724560); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4 S1B Ythdc2-/- (merge) , 4 S1B Ythdc2-/- SYCP3

4K ©

Specimen 4K: postnatal day 14 (more

)
Note: Double labeled: red - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong	Regionally restricted	4K	High levels of expression in most tubules.

4L ©

Specimen 4L: postnatal day 14 (more

)
Note: Double labeled: red - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong	Regionally restricted	4L	Authors report expresion along entire length of chromosomes.

4M ©

Specimen 4M: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: red - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Weak		4M	Expression usually low.

4N ©

Specimen 4N: postnatal day 14; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: red - Sycp3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Weak	Punctate	4N	Expression often localized in punctate pattern.

5 S1I ©

Specimen 5-S1I: embryonic day 15.5 (more

)
Note: Triple labeled: red - Sycp3; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1I

5 S1J ©

Specimen 5-S1J: embryonic day 15.5 (more

)
Note: Double labeled: red - Sycp3; green - Ddx4 (assay MGI:6724548).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1J

5 S1K ©

Specimen 5-S1K: embryonic day 15.5 (more

)
Note: Double labeled: red - Sycp3; green - Ddx4 (assay MGI:6724548).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1K	Expression in some germ cells at E15.5

7A ©

7A'' ©

Specimen 7A: postnatal day 12 (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7A'' , 7A

7B ©

7B'' ©

Specimen 7B: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7B , 7B''

7C ©

Specimen 7C: postnatal day 12 (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7C

7D ©

Specimen 7D: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7D

7 S1A ©

7 S1A'' ©

Specimen 7-S1A: postnatal day 12 (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7 S1A'' , 7 S1A

7 S1B ©

7 S1B'' ©

Specimen 7-S1B: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: red - Sycp3; green - Cyclin A2 (Ccna2 assay MGI:6724564); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7 S1B , 7 S1B''

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/28/2024 MGI 6.13