Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:253477 Bailey AS, et al., The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife. 2017 Oct 31;6:e26116
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6724548
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	anti-VASA (AF2030)
Detection system:	Alexa Fluor

Results

1 S1A' ©

Specimen 1-S1A: postnatal day 10 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1A'

1 S1B' ©

Specimen 1-S1B: postnatal day 12 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1B'

1 S1C' ©

Specimen 1-S1C: postnatal day 14 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1C'

4 S1C ©

Specimen 4-S1C: postnatal day 12 (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		4 S1C

4 S1D ©

Specimen 4-S1D: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		4 S1D

5 S1E ©

Specimen 5-S1E: postnatal day 0.5 (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of ovary	Present		5 S1E

5 S1F ©

Specimen 5-S1F: postnatal day 0.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of ovary	Present		5 S1F	Expresion in premeiotic germ cells that appeared similar to those in wild type

5 S1J ©

Specimen 5-S1J: embryonic day 15.5 (more

)
Note: Triple labeled: green - Ddx4; red - Sycp3 (assay MGI:6724544).\; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1J

5 S1K ©

Specimen 5-S1K: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: green - Ddx4; red - Sycp3 (assay MGI:6724544).\; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1K

5M ©

Specimen 5M: embryonic day 15.5 (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5M

5N ©

Specimen 5N: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5N

5O ©

Specimen 5O: embryonic day 15.5 (more

)
Note: Double labeled: green - Ddx4; red - CyclinD1 (Ccnd1 assay MGI:6724562).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5O

5P ©

Specimen 5P: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - CyclinD1 (Ccnd1 assay MGI:6724562).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5P

5Q ©

Specimen 5Q: embryonic day 16.5 (more

)
Note: Double labeled: green - Ddx4; red - phospho-Histone H3 (Thr3).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		5Q

5R ©

Specimen 5R: embryonic day 16.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - phospho-Histone H3 (Thr3).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		5R

5 S2C ©

Specimen 5-S2C: postnatal day 12 (more

)
Note: Double labeled: green - Ddx4; red - Meioc (assay MGI:6724563).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5 S2C

5 S2D ©

Specimen 5-S2D: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Meioc (assay MGI:6724563).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5 S2D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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