Ythdc2^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ythdc2^{tm1b(EUCOMM)Hmgu}
• Name: YTH domain containing 2; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6110119
• Gene: Ythdc2 Location: Chr18:44961521-45022787 bp, + strand Genetic Position: Chr18, 23.81 cM
• Alliance: Ythdc2^{tm1b(EUCOMM)Hmgu} page
• IMPC: Ythdc2 gene page

Mutation origin

• Mutant Cell Line: HEPD0563_8_G03
• Germline Transmission: Earliest citation of germline transmission: J:253477
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 44972828 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 44974773. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). (J:253477)

Expression

In Mice Carrying this Mutation:

38 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ythdc2 Mutation: 58 strains or lines available

References

• Original: J:253477 Bailey AS, et al., The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife. 2017 Oct 31;6:e26116
• All: 1 reference(s)