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Gene Expression Data
Assay Details
Assay
Reference: J:306370 Harland LTG, et al., The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nat Cell Biol. 2021 Jan;23(1):61-74
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6721395
Gene symbol: Eomes
Gene name: eomesodermin
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1d: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Note: Fixed in 1% paraformaldehyde. Double labeled: green - Eomes reporter; red - Kdr (assay MGI:6721396).
Structure Level Pattern Image Note
TS11: blood island Present 1d green inset , 1d merge , 1d merge inset , 1d green
Specimen 1e: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Note: Fixed in 1% paraformaldehyde. Double labeled: green - Eomes reporter; red - Runx1 (assay MGI:6721397).
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Present 1e merge , 1e merge inset , 1e green , 1e green inset
Specimen 1f: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Note: Fixed in 1% paraformaldehyde. Double labeled: green - Eomes reporter; red - Eomes protein (assay MGI:6721392).
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Present 1f merge inset , 1f green inset , 1f green , 1f merge Reporter expression persists in this tissue longer than the endogenous protein.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory