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Gene Expression Data
Assay Details
Assay
Reference: J:306370 Harland LTG, et al., The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nat Cell Biol. 2021 Jan;23(1):61-74
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721392
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Eomes/Tbr2 (ab23345)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1b: embryonic day 6.5 (more )
Note: Early-streak stage embryo. Double labeled: green - Eomes; red - Brachyury (assay MGI:6721393).
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1b green , 1b merge Expression is seen in proximal epiblast/primitive streak cells.
TS9: primitive streak Present 1b merge , 1b green Expression is seen in proximal epiblast/primitive streak cells.
Specimen 1c: embryonic day 6.5 (more )
Note: Mid-streak stage embryo. Double labeled: green - Eomes; red - Brachyury (assay MGI:6721393).
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 1c green , 1c merge Expression is seen in proximal epiblast/primitive streak cells.
TS10: primitive streak Present 1c merge , 1c green Expression is seen in proximal epiblast/primitive streak cells.
Specimen 1f: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Note: Fixed in 1% paraformaldehyde. Double labeled: red - Eomes protein; green - Eomes reporter (assay MGI:6721395).
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Absent 1f merge , 1f red , 1f red inset , 1f merge inset Reporter expression persists in this tissue longer than the endogenous protein.
Specimen ED1a: embryonic day 6.5 (more )
Note: Mid-streak stage embryo. Double labeled: green - Eomes; red - Kdr (assay MGI:6721396).
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Extended Data 1a merge , Extended Data 1a green
Specimen ED1b: embryonic day 7.5 (more )
Note: Late-streak stage embryo. Fixed in 1% paraformaldehyde. Double labeled: green - Eomes; red - Kdr (assay MGI:6721396).
Structure Level Pattern Image Note
TS11: yolk sac Absent Extended Data 1b merge inset , Extended Data 1b green , Extended Data 1b green inset , Extended Data 1b merge
TS11: embryo mesoderm Present Extended Data 1b merge inset , Extended Data 1b merge , Extended Data 1b green inset , Extended Data 1b green
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
MGI 6.24 		The Jackson Laboratory