Rbfox3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:297547 Leca I, et al., A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse. PLoS Genet. 2020 Nov;16(11):e1009104
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6695169
Gene symbol:	Rbfox3
Gene name:	RNA binding protein, fox-1 homolog (C. elegans) 3

Antibody:	Anti-NeuN (clone A60; MAB377)
Detection system:	Secondary antibody coupled to Alexa Fluor 568
Assay notes:	Specimens were subjected to heat-mediated antigen retrieval.

Results

3J ©

3M ©

Specimen 3J/M: postnatal day 6; Tg(Nes-cre)1Atp/0 (more

)
Note: Sagittal section. Double labeled: red - Rbfox3; green - Calb1 (assay MGI:6695168)

Structure	Level	Image	Note
TS28: cerebellum Purkinje cell layer	Present	3M , 3J	Expression is present in neurons.
TS28: cerebellum external granule cell layer	Present	3M , 3J	Expression is present in neurons.
TS28: cerebellum internal granule cell layer	Present	3M , 3J	Expression is present in neurons.
TS28: cerebellum molecular layer	Present	3M , 3J	Expression is present in neurons.

3K ©

3N ©

Specimen 3K/N: postnatal day 6; Tuba1a^tm1.1Dak/Tuba1a⁺ (more

)
Note: Sagittal section. Double labeled: red - Rbfox3; green - Calb1 (assay MGI:6695168)

Structure	Level	Image	Note
TS28: cerebellum Purkinje cell layer	Present	3K , 3N	Expression is present in neurons.
TS28: cerebellum external granule cell layer	Present	3K , 3N	Expression is present in neurons.
TS28: cerebellum internal granule cell layer	Present	3K , 3N	Expression is present in neurons.
TS28: cerebellum molecular layer	Present	3K , 3N	Expression is present in neurons.

3L ©

3O ©

Specimen 3L/O: postnatal day 6; Tuba1a^tm1.1Dak/Tuba1a⁺, Tg(Nes-cre)1Atp/0 (more

)
Note: Conditional mutant. Sagittal section. Double labeled: red - Rbfox3; green - Calb1 (assay MGI:6695168)

Structure	Level	Image	Note
TS28: cerebellum Purkinje cell layer	Present	3L , 3O	Expression is present in neurons. In the mutant their number is significantly increased in this layer relative to controls.
TS28: cerebellum molecular layer	Present	3L , 3O	Expression is present in neurons. In the mutant their number is significantly increased in this layer relative to controls.
TS28: cerebellum internal granule cell layer	Present	3L , 3O	Expression is present in neurons. In the mutant their number is significantly reduced in this layer relative to controls.
TS28: cerebellum external granule cell layer	Present	3O , 3L

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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