Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278249
Iwafuchi-Doi M, et al., The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev Biol. 2011 Apr 15;352(2):354-66
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6682146
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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anti-Sox2 (AF2018)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1Cb N2+/+:
embryonic day 7.25
(more  )
Specimen
1Cb N2+/+:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural plate |
Present |
Regionally restricted |
1C(b) N2+/+
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Expression was detected in the anterior neural plate.
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Specimen
1Cb N2-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Rr550tm1.1Knd/Rr550tm1.1Knd
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural plate |
Weak |
Regionally restricted |
1C(b) N2-/-
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Residual expression was seen in the anterior neural plate of the N2-/- embryo.
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Specimen
S2B N2+/+:
embryonic day 3.5
(more )
Note: Blastocyst.
Specimen
S2B N2+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Blastocyst.
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Specimen
S2B N2-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Rr550tm1.1Knd/Rr550tm1.1Knd
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Blastocyst.
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Analysis Tools
