Gene Expression Image Detail

Reference

Figure

ID

J:278249 Iwafuchi-Doi M, et al., Dev Biol. 2011 Apr 15;352(2):354-66

1

MGI:6681885

Image

Caption

A.(a) Whole mount in situ hybridization in comparison with enhancer N2-LacZ activity (b) at E6.5, E7.75 and E8.5. Bars, 200 um. C,D. (a) Whole mount in situ hybridization at stages E6.75, E7.5 and E7.75 wild-type (N2+/+) and homozygous knockout (N2-/-) embryos. Expression in the chorion is marked by asterisks, and that in the posterior half of the embryo by broken lines. (b) Immunofluorescent staining of E7.25 embryos. Bars, 200 um. E. qRT-PCR analysis of the embryonic portion of late egg cylinders at E7.25, comparing wild-type (N2+/+) and enhancer N2 knockout (N2-/-) embryos. epiblast (ep); anterior neural plate (an).

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2010.12.027 Dev Biol 352: 354-66, Iwafuchi-Doi M; Yoshida Y; Onichtchouk D; Leichsenring M; Driever W; Takemoto T; Uchikawa M; Kamachi Y; Kondoh H, The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Copyright 2011.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A(a) E6.5	MGI:6682144 (Sox2)
A(a) E7.75	MGI:6682144 (Sox2)
A(a) E8.5	MGI:6682144 (Sox2)
C(a) N2+/+ E6.75	MGI:6682144 (Sox2)
C(a) N2+/+ E7.5	MGI:6682144 (Sox2)
C(a) N2+/+ E7.75	MGI:6682144 (Sox2)
C(a) N2-/- E6.75	MGI:6682144 (Sox2)
C(a) N2-/- E7.5	MGI:6682144 (Sox2)
C(a) N2-/- E7.75	MGI:6682144 (Sox2)
C(b) N2+/+	MGI:6682146 (Sox2)
C(b) N2-/-	MGI:6682146 (Sox2)
D N2+/+ E6.75	MGI:6682145 (Sox3)
D N2+/+ E7.75	MGI:6682145 (Sox3)
D N2-/- E6.75	MGI:6682145 (Sox3)
D N2-/- E7.75	MGI:6682145 (Sox3)
E Sox2	MGI:6690245 (Sox2)
E Sox3	MGI:6690246 (Sox3)