Rr550^tm1.1Knd
Targeted Allele Detail

Summary

• Symbol: Rr550^tm1.1Knd
• Name: regulatory region 550; targeted mutation 1.1, Hisato Kondoh
• MGI ID: MGI:6682027
• Synonyms: Sox2^N2-, Sox2^tm2.1Knd
• Gene: Rr550 Location: Chr3:34700326-34700717 bp Genetic Position: Chr3, Syntenic
• Alliance: Rr550^tm1.1Knd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278249
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP and neomycin resistance gene cassette were placed upstream of the 538 bp sequence corresponding to the N2 (neural plate 2) enhancer located 3861 bp upstream of the gene and a second loxP site downstream. Cre-mediated recombination removed the selection cassette and enhancer. (J:278249)

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr550 Mutation: 0 strains or lines available

References

• Original: J:278249 Iwafuchi-Doi M, et al., The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev Biol. 2011 Apr 15;352(2):354-66
• All: 1 reference(s)