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Rr550tm1.1Knd
Targeted Allele Detail
Summary
Symbol: Rr550tm1.1Knd
Name: regulatory region 550; targeted mutation 1.1, Hisato Kondoh
MGI ID: MGI:6682027
Synonyms: Sox2N2-, Sox2tm2.1Knd
Gene: Rr550  Location: Chr3:34700326-34700717 bp  Genetic Position: Chr3, Syntenic
Alliance: Rr550tm1.1Knd page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:278249
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Modified regulatory region)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA loxP and neomycin resistance gene cassette were placed upstream of the 538 bp sequence corresponding to the N2 (neural plate 2) enhancer located 3861 bp upstream of the gene and a second loxP site downstream. Cre-mediated recombination removed the selection cassette and enhancer. (J:278249)
Expression
In Mice Carrying this Mutation: 17 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rr550 Mutation:  0 strains or lines available
References
Original:  J:278249 Iwafuchi-Doi M, et al., The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev Biol. 2011 Apr 15;352(2):354-66
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory