About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:296647 Wanner N, et al., DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. J Am Soc Nephrol. 2019 Jan;30(1):63-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6512074
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (ab15249)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3D - renal vesicle - fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: proximal renal vesicle Present 3D renal vesicle fl/fl
Specimen 3D - renal vesicle - fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: proximal renal vesicle Present 3D renal vesicle fl/fl; cre
Specimen 3D - comma-shaped body - fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: comma-shaped body Present Regionally restricted 3D comma-shaped body fl/fl Expression is seen in proximal regions.
Specimen 3D - comma-shaped body - fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: comma-shaped body Present Regionally restricted 3D comma-shaped body fl/fl; cre Expression is seen in proximal regions.
Specimen 3D - s-shaped body - fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: proximal segment of s-shaped body Present 3D s-shaped body fl/fl
Specimen 3D - s-shaped body - fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: proximal segment of s-shaped body Present 3D s-shaped body fl/fl; cre
Specimen 3D - immature glomerulus - fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: maturing glomerular tuft Present Regionally restricted 3D immature glomerulus fl/fl Expression is seen in proximal regions.
Specimen 3D - immature glomerulus - fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - Jag1 (assay MGI:6512076)
Structure Level Pattern Image Note
TS27: maturing glomerular tuft Present Regionally restricted 3D immature glomerulus fl/fl; cre Expression is seen in proximal regions.
Specimen S4C het: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - methylated cytosine
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Present S4c Het Expression is seen in podocytes.
Specimen S4C KO: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - methylated cytosine
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Present S4c KO Expression is seen in podocytes.
Specimen S7B fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Wt1; red - activated Casp3 (assay MGI:6512080)
Structure Level Pattern Image Note
TS27: early nephron Present S7b fl/fl
Specimen S7B fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - activated Casp3 (assay MGI:6512080)
Structure Level Pattern Image Note
TS27: early nephron Present S7b fl/fl; cre
Specimen S7D: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Wt1; red - activated Casp3 (assay MGI:6512080)
Structure Level Pattern Image Note
TS23: early nephron Present S7d
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/09/2025
MGI 6.24 		The Jackson Laboratory