Dnmt1^tm2Jae
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm2Jae
• Name: DNA methyltransferase 1; targeted mutation 2, Rudolf Jaenisch
• MGI ID: MGI:2156895
• Synonyms: 2loxp, Dnmt1^fl, Dnmt1^tm4Jae, Dnmt^2lox, Dnmt^lox
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm2Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66733
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Homologous recombination inserted a floxed CMV-hygromycin-thymidine kinase expression cassette downstream of exon 5 and placed a loxP site in intron 3. Cre-mediated recombination in ES cells excised the expression cassette and left loxP sites flanking exons 4 and 5, which encode the localization motifs and the entire catalytic domain of the protein. (J:66733)

Expression

In Mice Carrying this Mutation:

104141 assay results 15 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 108 strains or lines available

Notes

Mouse embryo fibroblasts derived from chimeric mice made with Dnmt1^{tm2Jae/tm4Jae} ES cells in which imprinting had been eliminated (J:104860) display a significantly faster growth rate with nearly half (47%) of cells in the G2 or M phase of the cell cycle. These MEF cells become spontaneously immortalized. The chimeric mice experience widespread tumorigenesis.

References

• Original: J:66733 Jackson-Grusby L, et al., Loss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation. Nat Genet. 2001 Jan;27(1):31-9
• All: 76 reference(s)