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Gene Expression Data
Assay Details
Assay
Reference: J:296647 Wanner N, et al., DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. J Am Soc Nephrol. 2019 Jan;30(1):63-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6512070
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-Six2 (11562-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2B fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Six2; red - methylated cytosine
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 2B fl/fl Expression is seen in the nephron-progenitor niche.
Specimen 2B fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - methylated cytosine
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 2B fl/fl; cre Expression is seen in the nephron-progenitor niche.
Specimen 4A fl/+; cre: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - pan-cytokeratin
Structure Level Pattern Image Note
TS23: cap mesenchyme Present 4A fl/+; cre Expression is seen in the nephron-progenitor niche.
Specimen 4A fl/fl; cre: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - pan-cytokeratin
Structure Level Pattern Image Note
TS23: cap mesenchyme Present 4A fl/fl; cre Expression is seen in the nephron-progenitor niche. The number of cells is comparable in the het and hom.
Specimen 4B fl/+; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - pan-cytokeratin
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 4B fl/+; cre Expression is seen in the nephron-progenitor niche.
Specimen 4B fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - pan-cytokeratin
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 4B fl/fl; cre Expression is seen in the nephron-progenitor niche. The number of cells is reduced in the hom compared to the het.
Specimen S4A: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - methylated cytosine; also stained with Hoechst
Structure Level Pattern Image Note
TS27: cap mesenchyme Present S4a Expression is seen in nephron progenitors.
Specimen S7A fl/fl: embryonic day 18.5; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Six2; red - BrdU
Structure Level Pattern Image Note
TS26: cap mesenchyme Present S7a fl/fl Expression is seen in nephron progenitors.
Specimen S7A fl/fl; cre: embryonic day 18.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Six2; red - BrdU
Structure Level Pattern Image Note
TS26: cap mesenchyme Present S7a fl/fl; cre Expression is seen in nephron progenitors. The number of these cells that are activity dividing is reduced in the mutant compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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