L1cam Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:150043 Bush JO, et al., Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev. 2009 Jul 1;23(13):1586-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6500854
Gene symbol:	L1cam
Gene name:	L1 cell adhesion molecule

Antibody:

anti-L1-CAM

Results

5A ©

Specimen 5A: embryonic day 16.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present		5A

5B ©

Specimen 5B: embryonic day 16.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present	Regionally restricted	5B	Authors report no difference in migration of corpus callosum axons compared with wildtype.

5C ©

Specimen 5C: embryonic day 16.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present		5C

5D ©

Specimen 5D: embryonic day 16.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present	Regionally restricted	5D	Authors report no difference in migration of corpus callosum axons compared with wildtype.

5E ©

Specimen 5E: embryonic day 17.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	5E	Authors report significant crossing of corpus callosum at this stage.

5F ©

Specimen 5F: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	5F	Axons of corpus callosum have stalled lateral to midline and have begun to form bundles.

5I ©

Specimen 5I: embryonic day 17.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	5I	Authors report significant crossing of corpus callosum at this stage.

5J ©

Specimen 5J: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	5J	Axons of corpus callosum have stalled lateral to midline and have begun to form bundles.

6A ©

6D ©

Specimen 6A/D: embryonic day 18.0 (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS26: corpus callosum	Present	Regionally restricted	6A , 6D	Expression in axons.

6E ©

6H ©

Specimen 6E/H: embryonic day 18.0 (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS26: corpus callosum	Present		6H , 6E

6I ©

6L ©

Specimen 6I/L: embryonic day 18.0 (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS26: corpus callosum	Present		6L , 6I

6M ©

6P ©

Specimen 6M/P: embryonic day 18.0 (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS26: corpus callosum	Present		6P , 6M

6Q ©

6T ©

Specimen 6Q/T: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	6Q , 6T	Co-expression with Efnb1within non-crossing corpus callosum axons.

6U ©

6X ©

Specimen 6U/X: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Triple labeled: green - L1cam; blue - Gfap (assay MGI:6500859); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present		6X , 6U

S3B ©

S3C ©

Specimen S3B/C: embryonic day 16.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present	Regionally restricted	S3B , S3C	Expression in corpus callosum axons that have not yet crossed at rostral position

S3E ©

S3F ©

Specimen S3E/F: embryonic day 16.5 (more

)
Note: Double labeled: green - L1cam; red - Gfap (assay MGI:6500859).

Structure	Level	Pattern	Image	Note
TS24: corpus callosum	Present	Regionally restricted	S3E , S3F	Crossing of corpus callosum at more caudal positions.

S5A ©

S5D ©

Specimen S5A/D: embryonic day 17.5 (more

)
Note: Triple labeled: green - L1cam; blue - Efnb1 (assay MGI:6500860); red - Rgs3 (assay MGI:6500864).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present	Regionally restricted	S5A , S5D	Co-expression with Rgs3 in axons of corpus callosum.

S5E ©

S5G ©

Specimen S5E/G: embryonic day 17.5 (more

)
Note: Double labeled: green - L1cam; red - Rgs3 (assay MGI:6500864).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present		S5E , S5G

S5H ©

S5J ©

Specimen S5H/J: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: green - L1cam; red - Rgs3 (assay MGI:6500864).

Structure	Level	Pattern	Image	Note
TS25: corpus callosum	Present		S5J , S5H

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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