Gfap Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:150043 Bush JO, et al., Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev. 2009 Jul 1;23(13):1586-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6500859
Gene symbol:	Gfap
Gene name:	glial fibrillary acidic protein

Antibody:

anti-GFAP

Results

5A ©

Specimen 5A: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present	Regionally restricted	5A	Expression in glial wedge and midline zipper glia guidepost structures.

5B ©

Specimen 5B: embryonic day 16.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present	Regionally restricted	5B	Authors report initial formation of glial wedge and midline zipper glia guidepost structures are normal compared with wildtype.

5C ©

Specimen 5C: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present	Regionally restricted	5C	Expression in glial wedge.

5D ©

Specimen 5D: embryonic day 16.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present	Regionally restricted	5D	Authors report initial formation of glial wedge is normal compared with wildtype.

5E ©

5G ©

Specimen 5E/G: embryonic day 17.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS25: cerebral hemisphere white matter	Present	Regionally restricted	5G , 5E	Authors report well-extended glial wedge shape.
TS25: indusium griseum	Present	Regionally restricted	5E , 5G	Induseum griseum is dorsally localized.

5F ©

5H ©

Specimen 5F/H: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS25: cerebral hemisphere white matter	Present	Regionally restricted	5H , 5F	Distorted and reduced appearance of Gfap-expressing cells in glial wedge. Mislocalization of guidpost cells at midline in path where corpus callosum axons destined to migrate, as well as within forming Probst bundles of axons.
TS25: indusium griseum	Present	Regionally restricted	5F , 5H	Collapse of Gfap-expressing cells of induseum griseum associated with aberrant midline localization.

5I ©

Specimen 5I: embryonic day 17.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS25: cerebral hemisphere white matter	Present	Regionally restricted	5I	Authors report well-extended glial wedge shape.

5J ©

Specimen 5J: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS25: cerebral hemisphere white matter	Present	Regionally restricted	5J	Distorted and reduced appearance of Gfap-expressing cells in glial wedge.

6B ©

6D ©

Specimen 6B/D: embryonic day 18.0 (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS26: indusium griseum	Present		6D , 6B

6F ©

6H ©

Specimen 6F/H: embryonic day 18.0 (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS26: cerebral hemisphere white matter	Present	Regionally restricted	6F , 6H	Expression in glial wedge.

6J ©

6L ©

Specimen 6J/L: embryonic day 18.0 (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS26: indusium griseum	Present		6J , 6L

6N ©

6P ©

Specimen 6N/P: embryonic day 18.0 (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS26: cerebral hemisphere white matter	Present	Regionally restricted	6P , 6N	Expression in cells of glial wedge.

6R ©

6T ©

Specimen 6R/T: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS25: indusium griseum	Present	Regionally restricted	6T , 6R	Experssion in mislocalized induseum griseum cells.

6V ©

6X ©

Specimen 6V/X: embryonic day 17.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Triple labeled: blue - Gfap; green - L1cam (assay MGI:6500854); red - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS25: cerebral hemisphere white matter	Present	Regionally restricted	6V , 6X	Rostral positions display well-formed Gfap-expressing glial wedge.

S3A ©

S3C ©

Specimen S3A/C: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present	Regionally restricted	S3A , S3C	Rostral positions display well-formed Gfap-expressing glial wedge.

S3D ©

S3F ©

Specimen S3D/F: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - L1cam (assay MGI:6500854).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S3D , S3F
TS24: indusium griseum	Present		S3F , S3D

S4A ©

S4C ©

Specimen S4A/C: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4A , S4C

S4D ©

S4F ©

Specimen S4D/F: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4F , S4D

S4G ©

S4I ©

Specimen S4G/I: embryonic day 16.5; Efnb1^tm3.2Sor/Efnb1^tm3.2Sor (more

)
Note: Double labeled: red - Gfap; green - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4G , S4I

S4J ©

S4L ©

Specimen S4J/L: embryonic day 16.5; Efnb1^tm4.2Sor/Efnb1^tm4.2Sor (more

)
Note: Double labeled: red - Gfap; green - Efnb1 (assay MGI:6500860).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4J , S4L

S4M ©

S4O ©

Specimen S4M/O: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4O , S4M

S4P ©

S4R ©

Specimen S4P/R: embryonic day 16.5 (more

)
Note: Double labeled: red - Gfap; green - Ephb2 (assay MGI:6500861).

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Present		S4R , S4P

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