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Gene Expression Data
Assay Details
Assay
Reference: J:98918 Kassar-Duchossoy L, et al., Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev. 2005 Jun 15;19(12):1426-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6489363
Gene symbol: Pax7
Gene name: paired box 7
Antibody: anti-Pax7
Results
Specimen 1A Pax7: embryonic day 9.75 (more )
Structure Level Pattern Image Note
TS16: dermomyotome Present Regionally restricted 1A Pax7 Coexpression with Pax7 in almost all dermomyotomal cells. Preferentially expressed in central dermomyotome.
TS16: myotome Ambiguous 1A Pax7 Majority of myotomal cells are Pax7-negative before E10.5
Specimen 1D: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: dermomyotome Present Regionally restricted 1D Pax7-expressing cells released from central portion of demomyotome.
TS19: myotome Present Regionally restricted 1D Expression in cells entering myotome on dorsal side.
Specimen 1D Pax7: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1D Merge , 1D Pax7 Expressing cells become dispersed among differentiating myotomal cells.
Specimen 1F: embryonic day 11.5; Pax3Sp/Pax3Sp (more )
Note: Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1F Authors report exclusion with MyHc in myotome.
Specimen 1F Pax7: embryonic day 11.5; Pax3Sp/Pax3Sp (more )
Note: Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1F Merge , 1F Pax7 Authors report exclusion with MyHc in myotome.
Specimen 1G Pax7 (upper): embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Des (assay MGI:6489365).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1G Merge (upper) , 1G Pax7 (upper) Pax7-expressing cells dispersed amond differentiating myotomal cells. Majority of expressing cells excluded from myoblasts.
Specimen 1G Pax7 (lower): embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Myog (assay MGI:6489366).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1G Merge (lower) , 1G Pax7 (lower) Pax7-expressing cells dispersed amond differentiating myotomal cells. Majority of expressing cells excluded from differentiating cells.
Specimen 2D Pax7: embryonic day 15.5; Myf5tm2Tajb/Myf5tm2Tajb, Myf6tm1Tajb/Myf6tm1Tajb (more )
Note: Double labeled: red - Pax7; dark nuclei - Myf6 reporter (assay MGI:6489375).
Structure Level Pattern Image Note
TS23: skeletal muscle Present Regionally restricted 2D Merge (upper) , 2D Pax7 No coexpression with Mrf4 (Myf6) reporter.
Specimen 3A Pax7: embryonic day 14.0; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS22: trunk musculature Present Regionally restricted 3A Merge , 3A Pax7 Expression in trunk muscles.
Specimen 3B: embryonic day 16.5; Myf5tm1Tajb/Myf5tm1Tajb, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Triple labeled: red - Pax7; green - Des (assay MGI:6489365); blue - Hoechst.
Structure Level Pattern Image Note
TS24: intercostal muscle Present Regionally restricted 3B Authors report expression in cells associated with residual intercostal embryonic fibers in absence of myogenesis.
Specimen 3C Pax7: embryonic day 16.5; Myf5tm1Tajb/Myf5tm1Tajb, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489374).
Structure Level Pattern Image Note
TS24: intercostal muscle Present Regionally restricted 3C Pax7 , 3C Merge Coexpression in some muscle progenitor cells.
Specimen 3D Pax7 (right): embryonic day 14.5; Myf5tm1Tajb/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Casp3 (assay MGI:6489368).
Structure Level Pattern Image Note
TS22: intercostal muscle Present Regionally restricted 3D (right) , 3D (right) inset Coexpression in some muscle progenitor cells.
Specimen 4A Pax7: embryonic day 16.5 (more )
Note: Double labeled: red - Pax7; green - laminin.
Structure Level Pattern Image Note
TS24: trunk musculature Present Regionally restricted 4A Pax7 laminin , 4A Pax7 Expression in cells associated with muscle fiber.
Specimen 4B Pax7: embryonic day 18.5; Myf5tm1Pas/Myf5+ (more )
Note: Triple labeled: red - Pax7; green - laminin; green - PM-histone H3.
Structure Level Pattern Image Note
TS26: trunk musculature Present Regionally restricted 4B Pax7 PM-H3 Lam , 4B Pax7 Coexpression with PM-Histone3 in satellite cells.
Specimen 4C Pax7: postnatal newborn; Myf5tm1Pas/Myf5+ (more )
Structure Level Pattern Image Note
TS27: forelimb muscle Present Regionally restricted 4C Pax7 Expression in satellite cells from freshly isolated forelimb fiber.
Specimen 4D Pax7: embryonic day 17.0; Myf5tm1Pas/Myf5+, Pax3Sp/Pax3Sp (more )
Note: Double labeled: red - Pax7; dark nuclei - Myf5 reporter (assay MGI:6489371).
Structure Level Pattern Image Note
TS25: skeletal musculature Present Regionally restricted 4D Pax7 , 4D Pax7 Xgal Expression in satellite cells.
Specimen S1A Pax7 (left): embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: dermomyotome Present Regionally restricted S1A nlacZ/+ Pax7betagal , S1A nlacZ/+ Pax7 Expression in cells 'parachute" from central dermomyotome towards myotome.
Specimen S1A Pax7 (right): embryonic day 11.5; Myf5tm1Pas/Myf5tm1Pas (more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: dermomyotome Present Regionally restricted S1A nlacZ/nlacZ Pax7 , S1A nlacZ/nlacZ Pax7betagal Expression in many developmentally arrested muscle progenitor cells located dorsally and ventrally.
Specimen S1B Pax7: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - Pax3 (assay MGI:6489364).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted S1B Pax7 , S1B Merge Coexpression with Pax3 in cells released from central dermomyotome into myotome.
Specimen S1D Pax7: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; green - PM-H3.
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted S1D Pax7 , S1D Pax7 PM-H3 Coexpression with proliferation marker PM-Histone3.
Specimen S2 Pax7: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax7; dark nuclei - Myf5 reporter (assay MGI:6489371).
Structure Level Pattern Image Note
TS19: limb mesenchyme Present Regionally restricted S2_Pax7 Xgal , S2_Pax7 Expression in developing limb muscles.
Specimen not shown: embryonic day 14.5; Myf5tm1Tajb/Myf5tm1Tajb, Myod1tm1Jae/Myod1tm1Jae (more )
Structure Level Pattern Image Note
TS22: skeletal musculature Present Regionally restricted Expression in clusters in presumed muscle forming regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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