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Gene Expression Data
Assay Details
Assay
Reference: J:98918 Kassar-Duchossoy L, et al., Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev. 2005 Jun 15;19(12):1426-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6489364
Gene symbol: Pax3
Gene name: paired box 3
Antibody: anti-Pax3
Results
Specimen 1A Pax3: embryonic day 9.75 (more )
Structure Level Pattern Image Note
TS16: dermomyotome Present Regionally restricted 1A Pax3 Coexpression with Pax3 in almost all dermomyotomal cells. Higher expression in dorsal and ventral dermomyotomal lips.
TS16: myotome Ambiguous 1A Pax3 Majority of myotomal cells are Pax3-negative before E10.5.
TS16: dorsal root ganglion Present 1A Pax3
Specimen 1E: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax3; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: dermomyotome Present Regionally restricted 1E Coexpression with Myf5-reporter in developmentally arrested muscle progenitor cells in ventral dermomyotome lip.
TS19: myotome Present Regionally restricted 1E Authors report release of expressing cells into presumed myotomal space in absence of a myotome.
Specimen 1E Pax3: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: red - Pax3; green - Myf5 reporter (assay MGI:6489373).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 1E Merge , 1E Pax3 Authors report release of expressing cells into presumed myotomal space in absence of a myotome.
Specimen 2C Pax3: embryonic day 10.5; Myf5tm1Tajb/Myf5tm1Tajb, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax3; red - Myf6 (assay MGI:6489367).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 2C Merge , 2C Pax3 Coexpression with Mrf4 (Myf6) in nucleus of embryonic progenitor in dermomyotome.
Specimen S1B Pax3: embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: green - Pax3; red - Pax7 (assay MGI:6489363).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted S1B Pax3 , S1B Merge Coexpression with Pax7 in cells released from central dermomyotome into myotome.
Specimen S1C Pax3 (left): embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: green - Pax3; red - Ki67 (Mki67 assay MGI:6489369).
Structure Level Pattern Image Note
TS19: dermomyotome Present S1C Merge (left) , S1C Pax3 (left)
TS19: neural tube Present S1C Merge (left) , S1C Pax3 (left)
Specimen S1C Pax3 (right): embryonic day 11.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: green - Pax3; red - Ki67 (Mki67 assay MGI:6489369).
Structure Level Pattern Image Note
TS19: dermomyotome Present S1C Merge (right) , S1C Pax3 (right)
Specimen S3A Pax3: embryonic day 14.5; Myf5tm1Tajb/Myf5tm1Tajb, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: red - Pax3; green - Des (assay MGI:6489365).
Structure Level Pattern Image Note
TS22: skeletal musculature Present Clusters S3A Pax3 , S3A Merge Expression in clusters in presumed muscle forming regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory