Myf5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:98918 Kassar-Duchossoy L, et al., Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev. 2005 Jun 15;19(12):1426-31
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6489374
Gene symbol:	Myf5
Gene name:	myogenic factor 5

Reporter:	GFP
Antibody:	anti-GFP

Results

3C GFP ©

3C Merge ©

Specimen 3C GFP: embryonic day 16.5; Myf5^tm1Tajb/Myf5^tm1Tajb, Myod1^tm1Jae/Myod1^tm1Jae (more

)
Note: Double labeled: green - Myf5 reporter; red - Pax7 (assay MGI:6489363).

Structure	Level	Pattern	Image	Note
TS24: skeletal musculature	Present		3C GFP , 3C Merge

3E GFP ©

3E Merge ©

Specimen 3E GFP: embryonic day 14.5; Myf5^tm1Tajb/Myf5⁺ (more

)
Note: Triple labeled: green - Myf5 reporter; red - Casp3 (assay MGI:6489368); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS22: skeletal musculature	Present		3E Merge , 3E GFP

S3B GFP ©

Specimen S3B GFP: embryonic day 16.5; Myf5^tm1Tajb/Myf5^tm1Tajb, Myod1^tm1Jae/Myod1^tm1Jae (more

)
Note: Triple labeled: green - Myf5 reporter; red - Des (assay MGI:6489365); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS24: skeletal musculature	Present	Regionally restricted	S3B Desmin GFP Hoechst , S3B GFP , S3B Desmin GFP	Expression in muscle progenitor cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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